227 results about "Sulfaclomide" patented technology

The invention relates to a method for detecting antibiotic residues in milk, and an application thereof, and belongs to the field of food safety. The invention discloses an online solid phase extraction-high performance liquid chromatography-mass spectrometry detection method in order to solve the detection problem of antibiotic residues in milk. The method allows five types of 33 veterinary drug residues comprising sulfanilamides, quinolones, beta-lactams, tetracyclines and chloramphenicols in the milk to be simultaneously detected. Influences of various factors on the reservation and selectivity of various antibiotics are optimized, so the method has the characteristics of high sensitivity, high recovery rate, high stability and high precision. The detected antibiotics can be qualitatively and quantitatively determined according to the reservation time and accurate mass numbers. The amounts of samples and reagents required and used in the method are small, the detection speed is fast, all operation processes are completed within 15.5min, and cost reduction and operating risk mitigation of dairy product processing enterprises are guaranteed.

A preparation method of sulfadoxine belongs to the field of sulfanilamide antimicrobial drug preparation. Cyclization reaction comprises the following steps of: firstly pouring a sodium methoxide solution into a reactive pan, then successively adding methanamide and methyl ethyl methoxymalonate, keeping warm, recovering methanol, cooling for crystallization, drying by centrifugation, discharging,and drying to obtain 5-methoxy-4,6-disodium dihydroxypyrimidine; Chlorination reaction comprises the following steps of: firstly putting phosphorus oxychloride into a reaction vessel for heating, adding 5-methoxy-4,6-disodium dihydroxypyrimidine into the reaction vessel to react, decompressing and recovering phosphorus oxychloride until the material is dry, cooling, adding trichloro ethylene withuniformly stirring, putting into a hydrolysis pan for hydrolyzation, collecting a trichloro ethylene layer after standing and delaminating, followed by a neutralization reaction, controlling pH value, washing, removing a water layer, recovering trichloro ethylene, and releasing crystals to obtain 5-methoxy-4,6-dichloropyrimidine. The preparation method provided by the invention can be used to guarantee the product purity, prolong the service life of equipment, avoid the damage to the environment and human body, reduce emission, and save energy, and accords with foreign pharmacopoeia standard requirements.

The invention discloses a sulfonamide compound and medicinal compositions thereof, and preparation methods and applications thereof. The sulfonamide compound can be used as a micro-molecular tubulin inhibitor, has an anti-microtubule effect in vitro, can induce the apoptosis of tumor cells in vitro, has an obvious inhibition effect on multidrug resistance cells in vitro, and has a substantial in-vivo oral antitumor activity. Additionally, the compound has the advantages of small molecular weight, simple synthesis, and small toxic side effects. The invention also provides medicinal compositions containing the sulfonamide compound and pharmaceutically acceptable salts thereof as an active component.

The invention relates to a method for preparing sulfadoxine, and belongs to the field of the preparation of sulfanilamide antibacterial medicaments. The method comprises the following processes of: performing methyl oxidation reaction and refining, wherein the process of performing the methyl oxidation reaction comprises the following step of: adding methanol solution into a reaction kettle with a stirrer, adding sodium hydroxide, stirring to perform reflux reaction, recovering methanol until the solution is dried, adding water, continuing to recover the methanol, pressing feed liquid into a decoloration pot, regulating the pH value of the feed liquid, adding the water and a decolorizer into the feed liquid, keeping the temperature and decolorizing; and after decolorizing, performing filter pressing on the feed liquid by a filter press to a precipitation pot for precipitating, regulating the pH value of the feed liquid again, centrifuging, dehydrating, spin-drying and discharging to obtain a sulfadoxine crude product. The process of refining comprises the following steps of: adding the water into a dissolution pot, adding the sulfadoxine crude product with heating and stirring, adding calcium to obtain liquid to be decolorated, decolorating, performing the filter pressing on the decolorated liquid by the filter press to a crystallization pot, regulating the pH value, centrifuging, dehydrating to obtain a filter cake, centrifuging, spin-drying, discharging and drying to obtain a sulfadoxine finished product. The method has the advantages of high yield, stable quality of the finished product and high purity, and residues of a harmful solvent carried in the previous reaction process are reduced.

The invention relates to a paper chip for detecting the residual antibiotic in milk, particularly to a paper chip detection method for the residues of five antibiotics such as tetracycline, chloramphenicol, sulfanilamide, quinolone and beta-lactam in milk. A purpose of the present invention is to provide a multi-channel detection paper chip. According to the present invention, the detection quantity, the detection index and the detection time are improved, the opening platform is provided, and the detection index on the paper chip can be adjusted according to the actual demand; and the results show that the paper chip has advantages of low cost, high sensitivity, platform opening, accurate result and the like, and provides a certain guidance significance for the development of the paper chip in the food safety detection field.

A preparation method of a traditional Chinese medicine lotion for treating redness-swelling type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotics and sulfanilamide medicaments are generally used for the treatment of redness-swelling type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention is that the preparation method comprises the following steps: soaking the following 28 herbs of polygonum hydropiper, common lantana leaf, sedum, shrubby breynia, phymatopsis hastata, herba patriniae, changium smyrnioide, hemsleya macrosperma, rhizoma anemarrhenae, radix tinosporae, nasturtium, honeysuckle, bidens biternata, houttuynia, herba lespedezae cuneatae, hypericum perforatum, sedum sarmentosum bunge, sagina subulata, lycoris, patience dock root, radix sophorae flavescentis, balsam pear, folium llicis latifoliae, elephantopus scaber, gentrin knotweed, saxifrage, solanum nigrum and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating redness-swelling type cellulitis. The advantages are that the prepared traditional Chinese medicine is less in toxic and side effect, short in treatment course, and high in cure rate, and can also prevent adverse effect caused by western medicine administration.

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating erosive type chronic cervicitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating erosive type chronic cervicitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 29 kinds of herbs consisting of verbena, Galium aparine, dysosma versipellis, wikstroemia indica, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, Pennywort Herb, Rohdea japonica Roth, Aster ageratoides, radix sophorae tonkinensis, edible tulip, senecio scandens, Huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, sodaapple nightshade herb, radix scutellariae, coptidis rhizome, cortex phellodendri, Broomjutre Sida Herb, ovateleaf holly bark, starwort root, Chinese Iris seed and liquorice in water for soaking, then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of simple preparation, small toxic and side effect, short treatment course and high cure rate.

The invention belongs to the field of antibiotic detection, and relates to a method for analyzing and determining four sulfonamide antibiotics in a water sample. A negative pressure suction filtrationmembrane solid phase extraction technology is utilized, and a metal organic framework material NH2-MIL-101(FE) membrane solid phase extraction-high performance liquid chromatography method is adoptedto determine four antibiotics such as sulfadiazine, sulfamethazine, sulfachloropyridazine and sulfamethoxazole in an environmental water sample. The method is simple, convenient, rapid and sensitiveto operate, and has the advantages of low detection limit, accuracy and good reproducibility; and moreover, the raw materials are cheap and easy to obtain, the material preparation process is simple,the reaction conditions are mild, the application environment is friendly, and the market prospect is wide.

The invention provides a sulfonamide antibiotic synergistically degraded bacteria and an application thereof. The sulfonamide antibiotic synergistically degraded bacteria comprise arthrobacterium P27and nocardioides N27, wherein the arthrobacterium P27 is preserved in China General Microbiological Culture Collection Center on December 03, 2019, and the preservation number is CGMCC No. 19070; andthe nocardioides N27 is preserved in the China General Microbiological Culture Collection Center on December 03, 2019, and the preservation is CGMCC NO. 19071. According to the technical scheme, compounding of the two strains of bacteria can synergistically complete degradation of sulfamethoxazole and an azacyclo product 3-amino-5-methylisoxazole; and in addition, the strain P27 can degrade one side of benzene rings of various sulfonamide antibiotics, the degradation substrate spectrum is wide, and a new idea and a new strain resource are provided for deep removal of the sulfonamide antibiotics.

The invention discloses a bionic enzyme linked immunosorbent assay method capable of rapidly determining sulfonamide antibiotic residues. The bionic enzyme linked immunosorbent assay method is characterized by comprising the following steps: (1) adding cyclohexane and Triton X-100 into a two-mouth flask; after magnetically stirring, adding quantum dots, tetraethyl orthosilicate and ammonia water in sequence; after magnetically stirring, adding a template molecule solution and a functional monomer; at room temperature, carrying out polymerization reaction to remove sulfonamide antibiotics; dispersing into an ethanol phosphate solution to obtain a molecular imprinting-quantum dot nanostructure artificial antibody solution; (2) adding the molecular imprinting-quantum dot nanostructure artificial antibody solution and a sample extracting solution into each enzyme linked immunosorbent assay hole in sequence; incubating an enzyme linked immunosorbent assay plate at room temperature for 10min and reading a numerical value under an emission wavelength of 605nm by utilizing an enzyme linked immunosorbent assay instrument; calculating the content of the sulfonamide antibiotics in the extracting solution through a standard curve according to a fluorescence value. The bionic enzyme linked immunosorbent assay method has the advantages of high specificity, high sensitivity, simplicity in synthesis, good stability and short detection time.

The invention discloses a preparation method and an application of a dodecahedron monatomic iron Fenton catalyst. The preparation method comprises the following steps: dissolving ferric acetylacetonate, zinc nitrate hexahydrate and dimethylimidazole in methanol, ultrasonically dissolving and uniformly stirring, transferring the obtained reaction mixture into a reaction kettle, and preparing an MOFs-loaded iron-based material precursor through solvothermal reaction; and calcining at a high temperature in an argon atmosphere to obtain the dodecahedral monatomic iron catalyst with a large specific surface area, and applying the dodecahedral monatomic iron catalyst to a heterogeneous Fenton reaction to realize efficient degradation of sulfonamide antibiotics. The catalyst has the characteristics of good recycling performance, wide pH application range (3-8), simple preparation method and the like, has higher application value, and can be used for treating antibiotic wastewater.

The invention discloses a mixed microbial agent for degrading sulfonamide antibiotics in sewage and a preparation method and application thereof, belonging to the technical field of environmental microorganisms. The mixed microbial agent for degrading sulfonamide antibiotics in sewage is formed by mixing arthrobacter sp. YL1 and pseudomonas sp. YL2 in a volume ratio of 1: (2-3). The mixed microbial agent is used for treating sulfonamide antibiotics in sewage or degrading organic matters in sewage. The arthrobacter sp. YL1 and the pseudomonas sp. YL2 are separated from each other in the invention can use sulfonamide antibiotics as a unique carbon source for growth and can realize rapid and efficient degradation of the sulfonamide antibiotics; and compared with a single strain, the double-strain interaction microbial agent system has higher removal efficiency, more thorough removal amount and a more stable community structure in the process of practical application, and is very criticalto biological treatment of sulfonamide antibiotics in water. The mixed microbial agent of the invention is applied to the field of antibiotic degradation.

The invention provides a method for detecting antibiotic residues in a water body environment based on the solid phase extraction technology and the liquid chromatography tandem mass spectrometry technology. In terms of detection effect, the signal-to-noise ratio is improved by optimizing the mass spectrum conditions, the peak shape is improved, and a lower detection limit of the method of a sample reaches 0.08 to 9.37ng/L; in terms of sample pretreatment, only 50mL water sample is required, thereby shortening the pretreatment time; in terms of the number of antibiotics and the detection time,42 kinds of antibiotics in 5 major categories, such as fluoroquinolones, sulfonamides, macrolides, chloramphenicols and lincomamides, can be separated and detected at one time just within 12min by optimizing the chromatographic conditions.

The invention provides a rapid detection method of sulfathiazole in an animal body, and a Raman characteristic peak of sulfathiazole is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. According to the method, the lowest detection concentration of sulfathiazole in an animal body is 1 mg/L. According to the method, the sulfonamide antibiotic residues in the animal body are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide thiazole residues in the animal body.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating urethral orifice burning type senile vaginitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating urethral orifice burning type senile vaginitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 38 kinds of herbs consisting of tartarian buckwheat root and rhizome, dandelion, dysosma versipelis, wikstroemia indica, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, Pennywort Herb, Rohdea japonica Roth, Aster ageratoides, radix sophorae tonkinensis, edible tulip, senecio scandens, Huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, ardisia crenata, centella asiatica, Polygonum hydropiper, sugarcane, HlblSClls llutabilis, sophora flavescens, balsam pear, ilex latifolia thumb, Elephantopus scaber, polygonum cuspidatum, saxifrage, folium phyllostachys and liquorice in water for soaking,then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of small toxic and side effect, short treatment course and high cure rate.

The invention belongs to the technical field of materials, and discloses a method for synthesizing [H2Nmim][NTf2]@UiO-66-Br nanocomposite material, wherein the material comprises bromine-functionalized Zr-MOFs(UiO-66-Br) and an amino-functional imidazole-based ionic liquid ([H2Nmim][NTf2]) supported thereon. The invention also discloses application of the nanocomposite material for the extractionand enrichment of sulfonamide antibiotics in a water sample prepared by dispersive solid phase microextraction technology (DSPME). The nanocomposite material of the invention has been successfully applied to the extraction and analysis of sulfonamide antibiotics, which can be completed in a short time using only a small amount of adsorbent and samples, and has the characteristics of fast, sensitive, efficient and economical application. The invention can also screen, design and regulate the adsorbent according to the structure of the target analyze, provides a new idea for the analysis of other environmental pollutants, and has important significance for protecting the health and safety of the public and the environment.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating stomach-yin deficiency type senile vaginitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating stomach-yin deficiency type senile vaginitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 25 kinds of herbs consisting of radix glehniae, black-bone chicken, fructus mori alba, radix scrophulariae, asparagus fern, dendrobium, fructus lycii, ligustrum lucidum ait, tortoise plastron, carapax trionycis, black sesame, black soybean, herba hyperici japonica, gentian, Polygonum hydropiper, sugarcane, HlblSClls llutabilis, sophora flavescens, balsam pear, ilex latifolia thumb, Elephantopus scaber, polygonum cuspidatum, saxifrage, ardisia crispa and licorice in water for soaking, then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of small toxic and side effect, short treatment course and high cure rate, and can avoid the adverse effects of applying western medicines.

The invention provides a sulfanilamide micromolecule surface modified polyamide composite membrane and a preparation method thereof. The polyamide composite membrane comprises an ultra-filtration bottom membrane and a micromolecule modified polyamide layer, the polyamide layer covers the surface of the ultra-filtration bottom membrane, and functional groups of the small molecules are amino and sulfamido. According to the sulfanilamide micromolecule surface modified polyamide composite membrane, easily available functional micromolecule monomers with amino and sulfanilamide functional groups are grafted to the surface of the polyamide composite membrane through a secondary interface polymerization method to obtain a modified membrane, the hydrophilicity of the surface of the membrane is improved by utilizing the hydrophilicity of sulfanilamide groups, so that the mass transfer of water molecules is accelerated, the purposes of high flux and pollution resistance are achieved, and the water flux of the modified membrane can be increased by 38-65% compared with that of an unmodified polyamide membrane on the premise of keeping high rejection rate; and in addition, the existence of thesulfonamide groups provides more active N-chloro groups, so that the active chlorine resistance of the polyamide composite membrane is also improved.

A preparation method of a traditional Chinese medicine lotion for treating phyma type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotics and sulfanilamide medicaments are generally used for the treatment of phyma type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention is that the preparation method comprises the following steps: soaking the following 29 herbs of semen calonyctionis muricati, galium aparine, dysosma versipellis, wikstroemia indica, herba saururi, radix berberidis, folium isatidis, big garden euphorbia herb, big herba lysimachiae, rohdea japonica root, aster ageratoides, subprostrate sophora, rhizoma pleionis, groundsel, lycopodium serratum, stephania, small garden euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, ilex pubescens, radix scutellariae, coptis, phellodendron, broomjutre sida herb, ovate leaf holly bark, radices stellariae dichotomae, common lantana leaf and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating phyma type cellulitis. The advantages are that the prepared traditional Chinese medicine is short in treatment course, and high in cure rate, and can also prevent adverse effect caused by western medicine administration.

The invention discloses preparation and application of a modified straw fiber adsorption material. The preparation method includes the steps of: 1) crushing straw, then immersing the crushed straw inalkali liquor, and performing separation; 2) carrying out etherification reaction and amination reaction successively in the treated straw sample; and 3) adding a phosphorylation agent into the obtained product for phosphorylation treatment, thus obtaining the modified straw fiber adsorption material. Specifically, the phosphorylation agent adopted in step 3) is a mixed solution containing paraformaldehyde and phosphorous acid. The method has the characteristics of mild reaction conditions, simple operation, and cheap and easily available raw materials. The adsorption material prepared by themethod has a good adsorption effect on sulfonamide antibiotics.

A preparation method of a traditional Chinese medicine lotion for treating blood stasis type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotics and sulfanilamide medicaments are generally used for the treatment of blood stasis type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention isthat the preparation method comprises the following steps: soaking the following 37 herbs of impatiens, leeches, fructus polygoni orientalis, humifuse euphorbia herb, rhizoma corydalis, native copper, artemisia anomala, safflower, azaleas, hematoxylon, Chinese honeylocust spine, myrrh, caulis spatholobi, frankincense, herba lycopi, selaginella, acronychia pedunculata, rhizoma polygoni paleacei, motherwort fruit, winged euonymus twig, turmeric, peach kernel, trumpet creeper, cloves, fiveleaf akebia fruit, star anise, murraya, aspongopus, sword bean, elecampane, toosendan fruit, mysorethorn, combined spicebush root, spikenard, ambergris, grubs and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating blood stasis type cellulitis. The advantages are that the prepared traditional Chinese medicine is simple in preparation, less in toxic and side effect, short in treatment course, and high in cure rate.

The invention provides a novel method for rapidly detecting and calculating content of sulfanilamide by detecting luminous intensity of sulfanilamide medicament imaging and establishing working curves, in order to solve the problems existed in the prior art for sulfanilamide medicament detection. The method can be used for accurately and economically detecting contents of sulfanilamide medicaments in animal food, especially is suitable for detecting contents of four sulfanilamide medicaments including sulfathiazole, sulfapyridine, sulphadiazine and sulfamerazine. The invention provides applications of the accurate, economic, high efficient, rapid and portable determination of residuals of the four sulfanilamide medicaments in animal food.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating toxic-heat type chronic cervicitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating toxic-heat type chronic cervicitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting herbs consisting of abrus cantoniensis hance, caulis lonicerae, Mahonia fortunei, saururus chinensis, daghestan sweetclover herb, coffee senna, common alstonia bark leaf, lophantherum gracile, butterflybush flower, mung bean, dandelion, sedum, herb of common corydalis, shrubby breynia, small-leaved desmodium, wintercherry, Jeffersonia dubia, uniflower swisscentaury root, olive, small-leaved lemmaphyllum, acanthaceae, gamboge, potentilla disclor bunge, indigo naturalis, spirifer, skink, nericum indicum, waxgourd peel, phaseolus calcaratus, Zanthoxylum bungeanum Maxim, stigma maydis, bottle gourd peel, dayflower, Polygonum aviculare, pink herb, caulis akebiae, Tetrapanax papyriferus and liquorice in water for soaking, then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of small toxic and side effect, short treatment course and high cure rate.

The invention provides a rapid detection method of sulfadiazine in an animal body, and a Raman characteristic peak of sulfadiazine is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and a good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. The lowest concentration of sulfadiazine in the animal body detected by the method is 2 mg/L. According to the method, the sulfonamide antibiotic residuesin the swine urine are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide antibiotic residues in the swine urine.