Monopterus albus growth prolactin antiserum as well as preparation method and application thereof

Abstract

The invention discloses a ricefield eel growth prolactin antiserum and a preparation method and application thereof.The preparation method includes the steps that expression plasmids containing nucleic acid molecules shown in SEQ ID NO: 4 are constructed, bacteria are transfected to obtain recombinant bacteria, inducible expression is conducted through isopropyl-beta-D-thiogalactoside, separation and purification are conducted to obtain ricefield eel Sml antigen, and the ricefield eel growth prolactin antiserum is obtained. And conventionally immunizing a test animal with the Sml antigen of the ricefield eel, immunizing for three times, and collecting serum to obtain the ricefield eel growth prolactin antiserum. The method is simple to operate and efficient in preparation, and the prepared antiserum is high in titer which can reach 1: 4096000 or above, is high in specificity and cannot be interfered by other pituitary hormones.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a rice field eel growth prolactin antiserum and its preparation method and application. Background technique [0002] The pituitary gland of fish is an important endocrine organ, which secretes a variety of protein hormones and regulates the growth and development process. Somatoprolactin (Sml) is a protein hormone secreted by the pituitary gland, which plays an important role in fish metabolism, growth and reproduction. Rice field eel is one of the important characteristic freshwater aquaculture fishes in China, with an annual output of more than 300,000 tons, which has extremely high economic value. However, in related technologies, there are very few reagents for regulating the growth of rice field eel. [0003] In related technologies, there are very few reports on the preparation of fish Sml antiserum. The traditional method is mainly to obtain Sml antiserum by separ...

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Problems solved by technology

However, the steps of isolating Sml protein from pituitary homogenate are cumbersome and difficult, and the acquisition rate is low, and there are problems of large homology differences among different fish, especially for rice field eel, which is different from conventional fish species such as salmon. The homology is less than 80%, there are large differences in the sequence, and the physical and chemical properties are also different. Therefore, the prepared antiserum cannot be effectively used for the use of specific fish species

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