Phosphine-based ionic liquid modified nano composite material, preparation method thereof and application of nano composite material in enrichment of phosphorylated peptide

Abstract

The invention discloses a phosphino ionic liquid modified nano composite material and a preparation method thereof, and application of the material to enrichment of phosphorylated peptides in a biological sample containing the phosphorylated peptides. Comprising the following steps: (1) carrying out substitution reaction on a chloropropyl modified substrate material and polyethyleneimine (PEI), and fixing the PEI on the surface of the substrate material; and (2) carrying out quaternization reaction on the brominated alkyl-containing phosphonate and the PEI layer to obtain the ionic liquid modified layer with organic phosphine functionalization. And (3) fixing metal ions on the surface of the material through an ester group hydrolysis reaction and a metal ion complexation reaction. The functionalized material prepared by the invention has the advantages of large specific surface area, excellent hydrophilicity, high metal immobilization amount, excellent sensitivity, high specific selectivity, high size exclusion effect and excellent reusability. The nanocomposite is suitable for enrichment and purification of phosphorylated peptides of complex biological samples. The material has a wide application prospect in the fields of proteomics and biomedicine.

Description

technical field [0001] The invention belongs to the field of functional materials and life sciences, and specifically relates to a phosphine-based ionic liquid modified nanocomposite material. At the same time, the invention also relates to a preparation method of the material and its application in enriching phosphorylated peptides. Background technique [0002] In the 1990s, with the rapid development of genomics, the sequencing of the human genome was basically completed. People began to realize that the research based on the mRNA level cannot contain all the information of life, so the research on protein, the direct executor of life activities, has gradually become a hot spot. According to statistics, in mammalian cells, more than 30% of proteins will be phosphorylated to varying degrees. Protein phosphorylation is the most extensive post-translational modification in nature, and different post-translational modifications play different roles in the regulation of life ...

AI Technical Summary

Problems solved by technology

At present, according to the different mechanisms of phosphorylated peptide enrichment, a variety of functional materials have been synthesized to capture phosphorylated peptides or phosphorylated proteins. These traditional methods have solved the problem of phosphorylated peptide enrichment, but some problems still cannot It is very good to avoid, such as weak anti-interference, low detection sensitivity, low selectivity, poor ability to exclude interference from biological macromolecules, etc.

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