Animal cell culture method

Abstract

The invention discloses an animal cell culture method. The animal cell culture method comprises the following steps: step S01, putting a carrier column on which a folding carrier with a plurality of folding surfaces is placed into a first chamber of a reaction chamber and fixing; s02, adding a culture solution into the first chamber until the flow guide container and the carrier column are submerged; step S03, inoculating cells; step S04, starting cell culture, starting a pressure applying mechanism arranged in a second chamber, pressing culture liquid which enters the second chamber downwards from the upper end of the flow guide container into a carrier column, enabling the culture liquid to penetrate through a carrier under the action of pressure and then flow out of the carrier column into a first chamber, and performing cell culture; finally, the water flows into the second cavity through the flow guide container and is pressed and fed into the carrier column to form circulation; and step S05, after culture is finished, opening the reaction cavity, and integrally taking out the carrier column and the carrier from the first cavity. The cell culture proliferation multiple can be remarkably improved, cell death in the culture process is prevented, and large-scale culture of various animal cells can be achieved.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for culturing animal cells. Background technique [0002] Bioreactor is an important equipment in the pharmaceutical industry, and its function is to cultivate cells for medical experiments. [0003] Currently, commonly used bioreactors include fluidized bed reactors and fixed bed reactors. However, the current bioreactors, whether they are fluidized bed reactors or fixed bed reactors, have the following problems: [0004] First of all, it is necessary to fill the bioreactor with granular cell culture carriers, which is not only time-consuming and laborious, but also difficult to operate. Moreover, due to the influence of cell metabolism and the distribution of cell culture medium, it is very difficult to expand the capacity of the bioreactor by using granular carriers for cell culture. [0005] At the same time, after the cell culture is completed, the gra...

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