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Composition and application thereof in stem cell culture

A composition and stem cell technology, applied in the field of stem cell culture, can solve the problems of high cost, high price, and restrictions on the wide-scale promotion of stem cell therapy, and achieve the effects of maintaining stemness, avoiding differentiation, and improving the ability of passage

Pending Publication Date: 2022-04-12
华科星河(北京)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When cells are exposed to serum and then placed under serum-free conditions, serum-derived contaminants may be carried with the cells, and this period of serum-exposed culture may ultimately limit their therapeutic use
Moreover, the existing serum-free medium is expensive, resulting in the cost of required materials several times higher than that of animal serum, which greatly limits the wide-scale promotion of stem cell therapy

Method used

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  • Composition and application thereof in stem cell culture
  • Composition and application thereof in stem cell culture
  • Composition and application thereof in stem cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Design experiments according to the formula in the following table:

[0067] Table 1 Medium formulations of each group

[0068]

[0069]

[0070] The preparation methods of each group of medium include:

[0071] Under the premise of using ultrapure water, the configuration of the medium can be carried out in three ways, and the preparation method has no effect on the results.

[0072] The first method: Dissolve the above substances in ultrapure water respectively, stir evenly without leaving any sediment and suspension, filter and sterilize through a 0.22μm filter membrane, and store at 4°C;

[0073] The second method: Use ultrapure water to prepare the concentrates of the above ingredients, filter and sterilize with a 0.22μm filter membrane, store at 4°C, and finally mix them in a certain proportion before use;

[0074] The third method: Dissolve the components that are easy to dissolve and store stable with ultrapure water to prepare a solution, filter and ...

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Abstract

The invention relates to the technical field of stem cell culture, in particular to a composition and application thereof in stem cell culture. The composition is formed by combining acetate, a pyruvate dehydrogenase kinase (PDK) specific inhibitor and an HMG-CoA reductase inhibitor, so that the capability of maintaining dryness of the mesenchymal stem cells is improved, and differentiation of the mesenchymal stem cells in the passage process is avoided. On the basis of the composition, multiple nutritional ingredients are further matched, the formed composition can provide sufficient nutrition for the mesenchymal stem cells, and the good multiplication capacity of the mesenchymal stem cells is still maintained in the absence of serum and in the presence of animal-derived components, and the dryness and batch stability of the mesenchymal stem cells in passage are maintained.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a composition and its application in stem cell culture. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of adult stem cells derived from mesoderm, which have the potential of self-renewal, multilineage differentiation and low immunogenicity. In vitro, mesenchymal stem cells can be induced to differentiate into epithelial, bone, cartilage, fat, nerve, heart and other tissue cells, which can promote blood vessel formation, protect nerves and cell replacement therapy. Mesenchymal stem cells also have potential clinical application prospects in clinical hematopoietic support, promotion of stem cell implantation, and immune regulation. [0003] Although there are abundant sources of mesenchymal stem cells, such as fat, umbilical cord blood, umbilical cord, placenta and other tissues, mesenchymal stem cells are adult stem cells, and the original concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 不公告发明人
Owner 华科星河(北京)生物科技有限公司
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