Hybridoma cell line secreting anti-human b7-h4 extracellular monoclonal antibody, anti-human b7-h4 monoclonal antibody and application thereof

A hybridoma cell line, B7-H4 technology, applied in the field of immune applications, can solve the problem of inability to resist tumor immune response, and achieve the effects of good passaging ability, high specificity and high affinity

Active Publication Date: 2020-09-29
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth mentioning that the inhibitory effect of B7-H4 on T cell activation cannot be reversed by the B7-CD28 co-stimulatory pathway, suggesting that the normal immune system of the body cannot block the immune escape mediated by B7-H4 by itself, so it cannot Autonomous generation of potent anti-tumor immune responses

Method used

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  • Hybridoma cell line secreting anti-human b7-h4 extracellular monoclonal antibody, anti-human b7-h4 monoclonal antibody and application thereof
  • Hybridoma cell line secreting anti-human b7-h4 extracellular monoclonal antibody, anti-human b7-h4 monoclonal antibody and application thereof
  • Hybridoma cell line secreting anti-human b7-h4 extracellular monoclonal antibody, anti-human b7-h4 monoclonal antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The hybridoma cell lines secreting extracellular monoclonal antibodies against human B7-H4 of this example, including five hybridoma cells that can produce high-titer monoclonal antibodies, are all deposited in the China Type Culture Collection, and the deposit address is Wuhan University , The preservation date is April 19, 2017, and the naming and preservation numbers are:

[0044] (1) Culture name (classified naming): hybridoma cell line 1C1H11, deposit number: CCTCC NO: C201758;

[0045] (2) Culture name (classification naming): hybridoma cell line 3E5G7, accession number: CCTCC NO: C201759;

[0046] (3) Culture name (classified naming): Hybridoma cell line 8H3C4, preservation number: CCTCC NO: C201760;

[0047] (4) Culture name (classified naming): Hybridoma cell line 12B2E1, deposit number: CCTCC NO: C201761;

[0048] (5) Culture name (classified naming): Hybridoma cell line 12H6G1, deposit number: CCTCC NO: C201762.

Embodiment 2

[0050] The anti-human B7-H4 monoclonal antibody of this embodiment is characterized in that: the anti-human B7-H4 monoclonal antibody is produced by the hybridoma cell line secreting anti-human B7-H4 extracellular monoclonal antibody according to claim 1 Of monoclonal antibodies.

[0051] Among them, the anti-human B7-H4 monoclonal antibody is a monoclonal antibody prepared by directly using eukaryotic expression of soluble human B7-H4 monomer protein as an antigen.

[0052] The anti-human B7-H4 monoclonal antibody can be used in the following aspects:

[0053] (1) Establish ELISA, chemiluminescence, immunofluorescence and other immunological methods to detect the concentration of soluble B7-H4 protein in human peripheral blood and other body fluids for immune diseases (including tumors, autoimmune diseases, infectious diseases, etc.) Clinical diagnosis, curative effect evaluation, prognostic judgment and pathogenesis research;

[0054] (2) It is used for the detection of cell surfac...

Embodiment 3

[0060] The application of the anti-human B7-H4 monoclonal antibody of this embodiment in the preparation of a drug for the detection of B7-H4 expression on the surface of human cells by flow cytometry.

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Abstract

The invention relates to the technical field of immune application, in particular to an anti-human B7-H4 extracellular monoclonal antibody secreting hybridoma cell strain, an anti-human B7-H4 monoclonal antibody and application thereof. The hybridoma cell strain comprises five hybridoma cells capable of generating high-potency monoclonal antibody and has good passaging capability. The antibody is the monoclonal antibody generated by the anti-human B7-H4 extracellular monoclonal antibody secreting hybridoma cell strain. The antibody can recognize a prokaryotic expression product, a eukaryotic expression product of human B7-H4 and expressed extracellular functional domain protein. The antibody is applied to immunological detection of the B7-H4, including detection of the B7-H4 expressed on the surfaces of the cells by a flow cytometry, detection of the B7-H4 expressed in tissues or the cells by an immunohistochemistry and a Western blot technology and detection of the soluble B7-H4 by an immunofluorescence technology, ELISA and chemiluminescence immunoassay; the antibody is also applied to antibody drug design.

Description

Technical field [0001] The present invention relates to the technical field of immunological applications, in particular to a hybridoma cell strain that secretes anti-human B7-H4 extracellular monoclonal antibodies and anti-human B7-H4 monoclonal antibodies and applications thereof. Background technique [0002] The dual-signal theory believes that T cell activation requires antigen signals and costimulatory signals. The former is transmitted by the TCR on the surface of T cells to recognize the MHC binding antigen peptides in the antigen-presenting cells, and the latter is caused by the interaction of costimulatory / co-inhibitory molecules and their ligands. Realize that the integration of the two signals can effectively induce T cell activation. Co-stimulatory / co-inhibitory molecules include a large group of known and unknown membrane molecules expressed on the surface of antigen-presenting cells and T cells, mainly including the immunoglobulin superfamily (Igsuperfamily, IgSF) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/28G01N33/68G01N33/574C12R1/91
CPCC07K16/2827C07K2317/33C07K2317/35C07K2317/92G01N33/57484G01N33/6872G01N2333/70532
Inventor 徐军发余诗炎庄泽刚张俊爱
Owner GUANGDONG MEDICAL UNIV
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