Hybridoma cell line secreting anti-human b7-h4 extracellular monoclonal antibody, anti-human b7-h4 monoclonal antibody and application thereof
A hybridoma cell line, B7-H4 technology, applied in the field of immune applications, can solve the problem of inability to resist tumor immune response, and achieve the effects of good passaging ability, high specificity and high affinity
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Embodiment 1
[0043] The hybridoma cell lines secreting extracellular monoclonal antibodies against human B7-H4 of this example, including five hybridoma cells that can produce high-titer monoclonal antibodies, are all deposited in the China Type Culture Collection, and the deposit address is Wuhan University , The preservation date is April 19, 2017, and the naming and preservation numbers are:
[0044] (1) Culture name (classified naming): hybridoma cell line 1C1H11, deposit number: CCTCC NO: C201758;
[0045] (2) Culture name (classification naming): hybridoma cell line 3E5G7, accession number: CCTCC NO: C201759;
[0046] (3) Culture name (classified naming): Hybridoma cell line 8H3C4, preservation number: CCTCC NO: C201760;
[0047] (4) Culture name (classified naming): Hybridoma cell line 12B2E1, deposit number: CCTCC NO: C201761;
[0048] (5) Culture name (classified naming): Hybridoma cell line 12H6G1, deposit number: CCTCC NO: C201762.
Embodiment 2
[0050] The anti-human B7-H4 monoclonal antibody of this embodiment is characterized in that: the anti-human B7-H4 monoclonal antibody is produced by the hybridoma cell line secreting anti-human B7-H4 extracellular monoclonal antibody according to claim 1 Of monoclonal antibodies.
[0051] Among them, the anti-human B7-H4 monoclonal antibody is a monoclonal antibody prepared by directly using eukaryotic expression of soluble human B7-H4 monomer protein as an antigen.
[0052] The anti-human B7-H4 monoclonal antibody can be used in the following aspects:
[0053] (1) Establish ELISA, chemiluminescence, immunofluorescence and other immunological methods to detect the concentration of soluble B7-H4 protein in human peripheral blood and other body fluids for immune diseases (including tumors, autoimmune diseases, infectious diseases, etc.) Clinical diagnosis, curative effect evaluation, prognostic judgment and pathogenesis research;
[0054] (2) It is used for the detection of cell surfac...
Embodiment 3
[0060] The application of the anti-human B7-H4 monoclonal antibody of this embodiment in the preparation of a drug for the detection of B7-H4 expression on the surface of human cells by flow cytometry.
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