Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria

A technology of tomato botrytis cinerea and piperine, which is applied in the field of agricultural biology and achieves the effects of wide acting sites, less resistance and low residue.

Pending Publication Date: 2022-05-13
QINGDAO AGRI UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the effectiveness of piperine on plant pathogens

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria
  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria
  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Botrytis cinerea, provided by Green Pesticide Research Institute of Qingdao Agricultural University, preserved at 4°C. Transfer the preserved strains to a culture plate for activation, put them in a constant temperature incubator and cultivate them at 25°C until the mycelia cover the entire plate, and use a puncher to punch a 5mm-diameter bacteria cake along the outer edge of the colony. spare.

[0029] 2. Accurately weigh 20 mg of piperine, dissolve it in DMSO, and prepare a 20,000 mg / L piperine stock solution.

[0030] 3. Determination of antibacterial activity by growth rate method.

[0031] Add the prepared piperine stock solution to the PDA medium (about 50°C) to form a drug-containing medium with a concentration of 200, 150, 100, 50, 25, and 12.5 mg / L, shake well and pour the plate . Take the culture medium with the same dose of DMSO as a control, inoculate d=5mm purely cultured hyphae discs of cinerea cinerea in the center of the plate, culture at a constan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of piperine in preparation of a bacteriostatic agent for preventing and treating tomato pathogenic bacteria. According to the application disclosed by the invention, the specific action part of the piperine in the process of inhibiting the botrytis cinerea is explored, and the piperine is verified to be capable of obviously inhibiting the spore growth and germination of the botrytis cinerea, changing the ultrastructure of thalli, inhibiting the enzyme activity of SOD, POD, CAT, SDH and NAD-MDH related to the thalli and obviously inhibiting the morbidity of tomato infection with the botrytis cinerea. Therefore, the piperine can effectively inhibit the growth of botrytis cinerea and is used for preparing a natural source bactericide.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to the application of piperine in the preparation of antibacterial agents for preventing and treating tomato pathogenic bacteria. Background technique [0002] Tomato is the vegetable with the second largest planting area in the world, and it is also an important economic crop in my country. Botrytis cinerea is a worldwide tomato disease. It is characterized by rapid transmission and serious damage. It is the most serious and frequent disease on tomatoes. The disease mainly damages fruits and causes fruit rot, and can also damage leaves and stems. Hehua has greatly affected the tomato production, and the production reduction can reach 20%-30% in severe cases, which brings huge economic losses to farmers. [0003] At present, there are many studies on the isolation and antibacterial effect of alkaloid compounds in plants at home and abroad, and reports have ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/40A01P3/00
CPCA01N43/40
Inventor 包华谢睿睿李康郝智慧
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com