Methods and reagents for nucleic acid sequencing and related applications

Abstract

The present technology generally relates to methods and associated reagents for providing error-corrected nucleic acid sequences. In particular, several embodiments relate to adaptor molecules including hairpin shapes and methods of using such adapters in double stranded sequencing and other sequencing applications. In some embodiments, a physically linked nucleic acid complex comprising both a first strand and a second strand can be amplified and independently sequenced in the same clone cluster on a sequencing surface.

Description

technical field [0001] The present technology generally relates to methods and related reagents for providing highly accurate (eg, error-corrected) nucleic acid sequences. In particular, several embodiments relate to adapter molecules comprising hairpin shapes and methods of using such adapters in double-strand sequencing and other sequencing applications. [0002] Cross References to Related Applications [0003] This application claims priority to and benefit from U.S. Provisional Patent Application No. 62 / 881,936, filed August 1, 2019, the disclosure of which is hereby incorporated by reference in its entirety. Background technique [0004] Double-stranded sequencing is an error-correcting method that achieves superior sequence accuracy by comparing sequence information derived from both strands of each double-stranded nucleic acid molecule. Concerning the efficiency of a double-stranded sequencing process or other high-accuracy sequencing modality, conversion efficienc...

AI Technical Summary

Problems solved by technology

In some cases, insufficient conversion efficiency may limit the utility of high-accuracy sequencing for applications where it would otherwise be well suited

For example, low transformati

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