Quantitative fingerprint spectrum detection method for components of common goldenrop capsule

A fingerprint and detection method technology, which is applied in the detection field of traditional Chinese medicine, can solve the problems such as the lack of quality detection methods for Xiaochaihu capsules, and achieve the effect of simple operation of the instrument

Pending Publication Date: 2022-05-31
浙江赞生药业有限公司
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  • Abstract
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Problems solved by technology

Therefore, in general, the current research on the quality detection methods of Xiaochaihu capsules is still very lacking.

Method used

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  • Quantitative fingerprint spectrum detection method for components of common goldenrop capsule
  • Quantitative fingerprint spectrum detection method for components of common goldenrop capsule
  • Quantitative fingerprint spectrum detection method for components of common goldenrop capsule

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[0031] It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present invention. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0032] It should be noted that the terminology used herein is for the purpose of describing specific embodiments only, and is not intended to limit the exemplary embodiments according to the present invention. Furthermore, it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they indicate the presence of features, steps, operations and / or combinations thereof.

[0033] 1. Experimental equipment and materials

[0034] 1.1 Instruments

[0035] Waters Acquity UPLC ultra-performance liquid chromatograph equipped with Acquity PDA detector, online degasser, quaternary pump, autosampler, co...

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Abstract

The invention discloses a quantitative fingerprint spectrum detection method for components of a common goldenrop capsule, and the method specifically comprises the following steps: (1) preparing a test solution and a reference substance mixed solution, taking four components of uridine, adenine, 5-hydroxymethylfurfural and guanosine as reference substances, respectively adding solvents for dissolving, and uniformly mixing to obtain the reference substance mixed solution; (2) analyzing and determining the two solutions prepared in the step (1) by adopting an ultra-high performance liquid chromatograph under the chromatographic conditions that an ultra-high performance liquid chromatography system is selected as an instrument, and a Waters ACQUITYUPLC HSS T3 chromatographic column (100mm * 2.1 mm, 1.8 [mu] m) is selected as a chromatographic column; carrying out isocratic elution by taking pure water as a mobile phase; and (3) determining common peaks and quantitative detection indexes according to 10 different batches of chromatograms, and evaluating the quality of the common bupleurum capsule according to the similarity and content of the fingerprints. The method can provide a scientific basis for quality control of the common goldenrop capsule.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a quantitative fingerprint detection method of components of Xiaochaihu capsules. Background technique [0002] Xiao Chai Hu Capsule is a compound preparation made from Bupleurum, Scutellaria, Licorice, Codonopsis, Jujube, Ginger, Ginger Pinellia through decoction, percolation and concentration. Symptoms include exchanges of cold and heat, fullness in the chest and flanks, loss of appetite, upset and vomit, bitterness in the mouth and dry throat, etc. In recent years, the research on Xiao Bupleurum preparations in academia has shown an increasing trend year by year. The 2020 edition of "Chinese Pharmacopoeia" (Part I) uses high performance liquid chromatography to determine the content of baicalin in Xiaochaihu Capsules for its quality control, and uses Bupleurum, Licorice, and Codonopsis to qualitatively identify these three herbs. However, Xiaochaihu Capsu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8606G01N30/8686G01N2030/027G01N2030/042
Inventor 龚行楚方爱军丁丰邵青邰艳妮周鹏虞焰钧李道超
Owner 浙江赞生药业有限公司
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