The invention discloses a method for detecting the copper content in rice based on the single-pulse laser-induced breakdown spectrum technology. The method includes the steps that 1, the rice is soaked with a copper solution, after the rice is dried at room temperature, grinding and sieving are carried out, and the rice is pressed into tablets through a tablet press; 2, laser-induced breakdown spectrum data X of various test samples is acquired; 3, flame-atomic absorption spectroscopy is selected to measure the real content Y of copper in the samples with various concentrations; 4, according to the corresponding relation between wavelengths and elements, original LIBS spectrum data is searched for wave crest intensities X1 corresponding to Cu I 324.754 nm and Cu I 327.396 nm; 5, after abnormal samples are removed, data X2 is obtained; 6, the data X2 is preprocessed, and a data set X3 is obtained; 7, by means of linear fitting, X3 serves as input, Y serves as output, and a linear regression model is established; 8, the linear regression model is used for carrying out quantitative analysis on the copper content in a certain number of samples, and the accuracy of the model is verified.