Immobilized enzyme composition for hexose production

A technology of immobilized enzymes and compositions, applied in the direction of immobilized enzymes, biofuels, oxidoreductases, etc., which can solve the problems that the cost of use hinders commercialization

Pending Publication Date: 2022-06-07
BONUMOSE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many enzymatic methods have been developed for commercial production (e.g., PCT applications WO 2018 / 169957, WO2017 / 059278 and WO 2018 / 112139), but when these methods are used for low-cost products (such as alternative sweeteners), the enzymatic The cost of use hinders the commercialization of these methods

Method used

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  • Immobilized enzyme composition for hexose production
  • Immobilized enzyme composition for hexose production
  • Immobilized enzyme composition for hexose production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Evaluation of enzyme carriers. The relative reaction rates of enzyme carriers for the production of tagatose enzyme compositions were evaluated, as well as their effect on enzyme stability. With these objectives in mind, the following enzyme carrier materials were evaluated: Four brands of phenolic matrix resins (DUOLITE TM A568, DUOLITE TM A561, DUOLITE TM PWA7, AmberLite TM FPA54); two brands of polystyrene resin (Lifetech TM ECR1640 and LifeTech TM ECR1504); two brands of polymethacrylate resin (Lifetech TM ECR8309M and Chromolite TM D6154); and a brand of controlled pore glass resin (EziG Opal TM ).

[0057] DUOLITE TM A568 (DuPont) ion exchange resin is a highly porous, granular, weakly basic anion exchange resin based on a cross-linked phenol formaldehyde polycondensate. Its hydrophilicity and controllable pore size distribution make it the most suitable resin for use as an enzyme carrier in many bioprocessing applications. DUOLITE TM The io...

Embodiment 2

[0075] Example 2 Effect of enzyme ratio on cascade activity rate. To evaluate the effect of varying the ratio of various immobilized enzymes on tagatose production, relative to DUOLITE prepared using the enzyme ratios described in Example 1 and Table 1 TM A568 - Immobilization Composition ("Example 1 Immobilization Composition"), Immobilized in DUOLITE by varying two immobilization composition formulations TM Dosage of αGP, PGM, PGI, F6PE, T6PP and 4-GT on A568. In contrast to the properties of the immobilized compositions of Example 1, their activity rates were evaluated. In one of the immobilized compositions with varying concentrations, the amount of enzyme used was based on the observed rate of activity of each enzyme relative to T6PP in solution, as shown in Table 4 ( Figure 1B ), where an equal amount of enzyme activity (μmol / min) was added to each enzyme. The activity ratio of this immobilized composition was 74% of the optimum ratio. Another immobilized compositio...

Embodiment 3

[0078] Example 3: Effect of enzyme distribution on one or more carriers. Using DUOLITE prepared in a single immobilization reaction as described in Example 1 TM A568 Method of immobilizing composition (19% αGP, 17% PGM, 3% PGI, 23% F6PE, 35% T6PP and 3% 4-GT) for the production of tagatose from maltodextrin, and activity using the following implementation method Rates were compared: (1) DUOLITE of core production enzymes (αGP, PGM, PGI and 4GT) TM DUOLITE of A568 Immobilization Composition and Special Enzymes for Tagatose Production (F6PE and T6PP) TM A568 mixture of immobilized compositions; and (2) a mixture of the following 6 separate immobilized enzyme preparations: DUOLITE TM A568 immobilized αGP, DUOLITE TM A568 immobilized PGM, DUOLITE TM A568 immobilized PGI, DUOLITE TM A568 immobilized 4GT, DUOLITE TM A568 immobilized F6PE and DUOLITE TM A568 immobilized T6PP. The ratios of all enzymes in (1) and (2) are the same as the reference DUOLITE TM The A568 immobiliz...

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Abstract

The invention relates to an immobilized enzyme composition for preparing hexose. The hexoses include, for example, tagatose, psicose, fructose, allose, mannose, galactose, antrose, talose, sorbose, gulose, idulose, and inositol. The invention also relates to an enzymatic process for the preparation of hexose from a saccharide by contacting a starch derivative with an immobilized enzyme composition of the invention.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims priority to US Application No. 62 / 875,321, filed July 17, 2019, and US Application No. 62 / 924,323, filed October 22, 2019, the entire contents of which are incorporated herein by reference. technical field [0003] The present invention relates to the preparation of hexose monosaccharides using immobilized enzyme compositions. More specifically, the present invention relates to a method for preparing D-hexose (or hexose) from saccharides such as polysaccharides, oligosaccharides, disaccharides, sucrose, D-glucose and D-fructose, comprising catalysis by immobilized enzymes One or more enzymatic steps of converting fructose 6-phosphate to hexose sugars. Background technique [0004] Hexoses are monosaccharides with six carbon atoms. Hexose sugars include, for example, tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gulose, idose, and inositol. Hexose sugars are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N9/90C12N11/02C12P19/02
CPCC12N9/90C12P19/02C12N11/02C12Y204/01001C12Y503/01009C12P19/24C12Y204/01025C12Y101/01017C12Y401/0204C12Y501/03C12Y504/02008C12Y504/02002C12Y503/01C12Y504/02C12Y504/02006C12N9/0006C12N9/88C12N9/96C12R2001/00C12N11/08C12N11/082C12N11/087C12N11/14C12N9/1051C12P19/18C12N9/246C12Y204/01024C12Y207/01144C12Y501/03015C12N11/091C12N9/107C12N9/16C12Y204/01018C12Y301/03
Inventor D·J·维歇莱茨基J·瓦格纳
Owner BONUMOSE INC
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