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Cauliflower low-generation inbred line cutting seed reserving method

A technology for cauliflower and inbred lines, which is applied in the fields of botanical equipment and methods, planting substrates, horticulture, etc., can solve the problems of long growth cycle, high technical requirements, and high production input costs, and achieves reduced breeding costs, good field growth, The effect of reducing the incidence of diseases

Pending Publication Date: 2022-06-24
宁波微萌种业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of obtaining seedlings through tissue culture has some problems, such as high production input costs, high technical requirements, and long growth cycles.

Method used

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  • Cauliflower low-generation inbred line cutting seed reserving method
  • Cauliflower low-generation inbred line cutting seed reserving method

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Embodiment

[0024] The invention discloses a method for cutting seeds of low-generation inbred lines of cauliflower, comprising the following steps:

[0025] 1. In the early stage of growth, the ability of cauliflower to produce cluster buds or lateral buds is weak. After cutting off the main curd, use 6-benzylaminopurine (6-AB) aqueous solution with cytokinin of 20mg / L and water-soluble compound. Fertilizer treatment promotes the occurrence and growth of lateral buds and cluster buds.

[0026] 2. In late December or early January, select the semi-lignified / lignified side branches or cluster buds of cauliflower as cuttings, and the cuttings can be 7-20cm long. Before cutting, make a 2-3cm incision at the base of the cutting to facilitate rooting. And remove most of the leaves to reduce transpiration and ease the seedlings. Before cutting, the incision is immersed in the rooting powder solution with a concentration of 500mg / L for 5 minutes, and the treated cuttings are inserted into the ...

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Abstract

The invention provides a cauliflower low-generation inbred line cuttage seed reserving method, which comprises the following steps of: treating a plant of which a main flower ball is cut off by cytokinin, and promoting differentiation and growth of lateral buds or cluster buds; selecting lateral buds or cluster buds of the cauliflower as cutting slips, treating the cutting slips with a substance for promoting root primordium differentiation, and cutting the cutting slips in a nutrition bowl filled with a substrate; preserving heat and moisture after cuttage, watering and fertilizing regularly, and obtaining complete plant seedlings after the cuttings root; the obtained plant seedlings are planted in a field, heat preservation and moisture preservation are paid attention to, and the seedling survival rate is increased; after the plants bloom, bagging and selfing are carried out, and selfing seeds are obtained. According to the method, the lateral branches or cluster buds of the cauliflower plants are used as cutting materials for cutting seedling raising, complete plants are obtained for seed reserving, main flower balls can be cut off to investigate the agronomic characters of the cauliflower, selfing line seeds can be harvested, the character investigation accuracy is improved, the breeding efficiency is improved, and the breeding cost is reduced.

Description

technical field [0001] The invention relates to the technical field of cauliflower seed saving, in particular to a method for cutting and saving seeds of a low-generation inbred line of cauliflower. Background technique [0002] Cauliflower, also known as cauliflower, cauliflower or cauliflower, originated from Crete in the eastern Mediterranean in Europe. Light yellow curd, etc., is one of the important vegetable crops. Cauliflower is an annual vegetable. It was introduced to my country in the 19th century and was first cultivated in a few coastal areas such as Fujian and Guangdong. In recent years, the cultivation area of ​​cauliflower has expanded rapidly, and it has developed from a traditional "fine vegetable" to one of the main vegetable crops in my country. At present, my country has become the world's first cauliflower producer and consumer. [0003] When screening excellent individual plants of cauliflower, it is often necessary to cut off the main cauliflower hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G22/00A01G24/15A01G9/22
CPCA01G2/10A01G22/00A01G24/15A01G9/222Y02A40/25
Inventor 吴新胜郭聚领许庆龙方辉
Owner 宁波微萌种业有限公司
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