Microfauna online culture and observation integrated device and use method thereof

A micro and animal technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems that the whole process of micro animal movement cannot be reflected, and micro animals cannot be cultivated and observed online. Achieve the effect of improving training and observation, maintaining free movement, and reducing interference

Pending Publication Date: 2022-07-12
ANHUI UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem that micro-animals cannot be cultivated and observed online in the prior art, and the point-to-surface observation cannot reflect the whole process of micro-animal movement, the present invention provides a device integrating on-line cultivation and observation of micro-animals, which can be used for long-term continuous cultivation and observation. Provide new technical methods for observing the movement, growth and reproduction of micro-animals in water treatment and related fields

Method used

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  • Microfauna online culture and observation integrated device and use method thereof
  • Microfauna online culture and observation integrated device and use method thereof
  • Microfauna online culture and observation integrated device and use method thereof

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Embodiment 1

[0041] combine Figure 1-Figure 3 As shown, an integrated device for online cultivation and observation of micro-animals in this embodiment includes an adjustable nutrition bottle 210 and a special glass slide 410 placed on the stage of the microscope 600. The position of the adjustable nutrition bottle 210 Higher than Specialty Slides 410. Specifically, in this embodiment, the top of the telescopic rod 112 is provided with a hook 113 , and the bottom of the telescopic rod 112 is fixed by a tripod 110 , wherein the adjustable nutrition bottle 210 is suspended on the hook 113 , and the adjustable nutrition bottle is attached to the adjustable nutrition bottle by adjusting the telescopic rod 112 210 to a suitable height. In this embodiment, the nutrient solution in the adjustable nutrient bottle 210 is input into the micro-incubator 430 of the special glass slide 410 through the water inlet hose 310 under the action of gravity, so as to provide the micro-incubators 430 with nut...

Embodiment 2

[0058] A method of using an integrated device for online cultivation and observation of micro-animals in this embodiment, the reaction device is basically the same as that described in Embodiment 1, the difference is that in this embodiment, the activated sludge that appears in the early stage of cultivation with sewage treatment is used. Trichoderma trigonata was used as the object of cultivation and observation, and the influence of the concentration of influent nutrient solution on its movement was analyzed. Trichinella ternata is a ciliated protozoa with small individuals and small free movement range in sewage treatment. Therefore, a special glass slide 410 equipped with a micro incubator 430 with a diameter of 0.4 mm was selected for cultivation and observation.

[0059] The COD concentration in the nutrient solution was gradually increased from 180.23 ± 40.25 mg / L to 780.33 ± 152.29 mg / L. Under the environment of 100 times magnification of the microscope, the movement be...

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Abstract

The invention discloses a microfauna online culture and observation integrated device and a use method thereof, and belongs to the technical field of microfauna observation and culture. The device comprises an adjustable nutrition bottle and a special glass slide placed on a microscope objective table, a plurality of miniature incubators are arranged on the special glass slide, the miniature incubators are communicated with one another, one ends of the miniature incubators are communicated with a liquid inlet distribution groove, and the other ends of the miniature incubators are communicated with a liquid outlet collection groove. The inlet end of the inlet liquid distribution tank is communicated with an adjustable nutrition bottle through a water inlet hose, and the outlet end of the outlet liquid collection tank is communicated with a waste liquid bottle through a drainage cotton thread. In order to solve the problems that in the prior art, microfauna cannot be cultured and observed on line, and point-to-surface observation cannot reflect the whole movement process of the microfauna, the invention provides the device integrating online culture and observation of the microfauna. And a new technical method is provided for long-time continuous culture and observation of movement, growth and reproduction of microfauna in water treatment and related fields.

Description

technical field [0001] The invention relates to the technical field of micro-animal observation and cultivation, and more particularly, to an integrated device for online cultivation and observation of micro-animals and a method for using the same. Background technique [0002] In the biological wastewater treatment process, micro-animal dominant species or community diversity indicators are often used to reflect the status of activated sludge / biofilm and the effect of wastewater treatment. big. Because the micro-animals are sensitive to the environment, their movement changes can well reflect the status of the activated sludge system. [0003] When the activated sludge / biofilm sample is placed on a microscope slide to observe the identification and movement speed of micro-animals, the micro-animals (measured in μm) are much smaller than the sample droplet volume (measured in mm). 3 ), which has a large range of motion and fast movement. When tracking and observing a micro...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/24C12M1/38C12M1/04C12M1/36C12M1/34C12N1/00G02B21/34
CPCC12M23/08C12M23/00C12M41/12C12M29/20C12M33/12C12M41/40C12M41/48C12M23/06C12M33/04C12M41/46C12N1/00G02B21/34Y02W10/10
Inventor 胡小兵李晶晶沈翼军陈红伟宋维维胡江楠李雪
Owner ANHUI UNIVERSITY OF TECHNOLOGY
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