Method for in-vitro germination and efficient rapid propagation of canna plant seeds

Abstract

The invention belongs to the technical field of ornamental plant tissue culture, and discloses a canna plant seed in-vitro germination and efficient rapid propagation method, which comprises the following steps: seed collection, seed disinfection, inoculation, in-vitro germination, aseptic seedling cutting and adventitious bud multiplication culture, test-tube seedling rooting culture, and test-tube seedling transplanting and domestication. A canna seed tissue culture and rapid propagation system is established, so that the canna breeding process can be accelerated, the acquisition of excellent hybrid clones is accelerated, and the rapid application of new varieties is promoted; meanwhile, an excellent material and technical system can be provided for in-vitro preservation of excellent hybrid germplasm, and the method has important significance on seedling breeding, production and cultivation of canna indica. The glass beads and the liquid culture medium are used as the culture medium, so that the use of agar and hormones is greatly reduced, and the culture cost is saved; meanwhile, the seed germination time of canna plants is greatly shortened, the breeding process is shortened, and the adventitious bud proliferation coefficient is increased.

Description

technical field [0001] The invention belongs to the technical field of ornamental plant tissue culture, and in particular relates to a method for in vitro germination and efficient and rapid propagation of Canna seeds. Background technique [0002] At present, Canna (Canna spp.) is a perennial bulbous flower of the Canna family Canna, native to America, and can be used in landscaping, polluted water purification, edible and disease treatment. It is an ornamental, ecological, medicinal and edible garden flower. . The traditional propagation method of canna mostly adopts division propagation, and the reproduction coefficient is extremely low, and long-term asexual propagation can easily cause virus accumulation in new plants, resulting in variety degradation, and the breeding speed of new varieties is extremely slow, which greatly limits the further application of canna; Fruit-bearing canna can be propagated by seeding, such as the large-flowered canna, canna, aquatic canna a...

AI Technical Summary

Problems solved by technology

The traditional reproduction method of canna mostly adopts ramet propagation, the reproduction coefficient is extremely low, and long-term asexual reproduction is easy to cause virus accumulation in new plants, resulting in variety degradation, and the speed of new variety breeding is extremely slow, which greatly limits the further application of canna; Fruit-bearing Canna can be propagated by seeding, such as Canna large flower, Canna taro, aquatic Canna and other populations, which are important parents for the improvement of modern Canna plant varieties, but the seed coats of Canna plants are dense and hard, and the emergence rate of conventional sowing is less than 2%

[0003] The problems and defects of the existing canna in vitro propagation technology are: the reproduction coefficient of the existing canna plant propagation method is extremely low, which is easy to cause virus accumulation in the new plant body and species degradation, and the seed coat is dense and hard, and the natural germination rate is low. Restricting the popularization and application of new hybrid varieties of canna

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