Method for simultaneously detecting tacrolimus and pimerolimus in cosmetics

Abstract

The invention discloses a method for simultaneously detecting tacrolimus and pimerolimus in cosmetics, and particularly relates to a qualitative and quantitative method for simultaneously detecting tacrolimus and pimerolimus in cosmetics by adopting high performance liquid chromatography tandem triple quadrupole mass spectrometry. The method is simple in pretreatment, good in precision and reproducibility, small in matrix effect and high in applicability, makes up for the blank of standards and inspection methods of the two components in the field of cosmetic inspection, establishes and perfects laws and regulations, can rapidly, accurately and efficiently qualitatively and quantitatively determine tacrolimus and pimerolimus illegally added in cosmetics, and has good application prospects. Powerful support is provided for supervision departments to timely discover forbidden components illegally added in cosmetics through powerful technical means, and life and property safety of people is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of cosmetic quality and safety detection, in particular to a method for simultaneously detecting tacrolimus and pimecrolimus in cosmetics. Background technique [0002] Tacrolimus is an immunosuppressive drug, a fermentation product isolated from Streptomyces, a macrolide antibiotic, a powerful new immunosuppressant, mainly by inhibiting interleukin The release of -2 (IL-2) comprehensively inhibits the effect of T lymphocytes, which is 100 times stronger than that of cyclosporine (CsA). FK506 also plays an active role in the treatment of autoimmune diseases such as atopic dermatitis (AD), systemic lupus erythematosus (SLE), and autoimmune eye disease. Pimecrolimus is a lipophilic anti-inflammatory derivative of ascomycin macrolactam that selectively inhibits the production and release of pro-inflammatory cytokines. In animal models of skin inflammation, pimecrolimus exhibits strong anti-inflammatory activi...

AI Technical Summary

Problems solved by technology

Tacrolimus and pimecrolimus are two commonly used dermatological drugs, which are mainly used for the treatment of moderate to severe atopic dermatitis in children and adults with poor response or intolerance, but both have a certain degree of Side effects, such as local itching, erythema, tingling, etc., and are prohibited for children under 2 years old, are prohibited ingredients that cannot be added to cosmetics, and the existing cosmetics-related standards have not formulated the test methods for these two ingredients

[0004] Although there are reports on the detection of tacrolimus and / or pimecrolimus in the existing literature and technology, they are all detection methods when they are used as medicines, such as tacrolimus and / or pimecrolimus in plasma and human metabolites. or concentration detection of pimecrolimus

Affected by different product types, cosmetics include creams, lotions, oils, waters and many other dosage forms. They have complex matrices and various ingredients, so they cannot be tested by existing drug testing methods.

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