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Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry

A technology of methcathinone and biological samples, which is applied in the field of drug detection in the field of criminal investigation, can solve the problems of complex biological sample system, troublesome detection work, and many interferences, and achieve high-efficiency qualitative and quantitative, good purification effect, and high detection accuracy Effect

Active Publication Date: 2015-08-12
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In the prior art, the liquid mass spectrometry method has been applied to the detection of cathinone, methcathinone, and 4-methylmethcathinone samples, but not for actual biological samples, because in actual investigation work, it is necessary to detect Most of the targets are biological samples, such as blood, urine, etc. There is no detection method for biological samples in the current method. Due to the characteristics of complex systems, many interferences, and difficult purification of biological samples, it brings great difficulties to the actual detection work. big trouble

Method used

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  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry
  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry
  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Liquid-mass spectrometry analyzes the method for cathinone, methcathinone, 4-methyl methcathinone in urine, it comprises the steps:

[0049] (1) Preparation of sample solution;

[0050] (2) Preparation of standard working solution;

[0051] (3) Setting of detection conditions;

[0052] (4) Liquid-mass spectrometry determination;

[0053] (5) Calculation of experimental results, where,

[0054]Step (1): Take three blank urine samples of healthy volunteers, add cathinone, methcathinone and 4-methyl methcathinone respectively to prepare urine sample 1 (cathinone concentration is 500ng / mL ), urine sample two (concentration of methcathinone is 500ng / mL) and urine sample three (concentration of 4-methyl methcathinone is 500ng / mL), respectively, according to the volume ratio of sample and water of 2:1 After proportional dilution, add protein precipitating agent acetonitrile, protein precipitating agent is added until no precipitation is formed in the sample, then after ult...

Embodiment 2

[0075] Liquid-mass spectrometry analyzes the method for cathinone, methcathinone, 4-methyl methcathinone in blood, and it comprises the steps:

[0076] (1) Preparation of sample solution;

[0077] (2) Preparation of standard working solution;

[0078] (3) Setting of detection conditions;

[0079] (4) Liquid-mass spectrometry determination;

[0080] (5) Calculation of experimental results, where,

[0081] Step (1): Take three blank blood samples from healthy volunteers, add cathinone, methcathinone and 4-methyl methcathinone respectively to prepare urine sample 1 (cathinone concentration is 500ng / mL) , Urine sample 2 (the concentration of methcathinone is 500ng / mL) and urine sample 3 (the concentration of 4-methyl methcathinone is 500ng / mL), after dilution with water at a volume ratio of 1:1, Add the protein precipitant acetonitrile, the protein precipitant is added until no precipitation is formed in the sample, then ultrasonically oscillate for 15 minutes, then centrifuge...

Embodiment 3

[0088] The method for analyzing cathinone, methcathinone, 4-methylmethcathinone and common adulterants thereof in blood by liquid chromatography-mass spectrometry comprises the following steps:

[0089] (1) Preparation of sample solution;

[0090] (2) Preparation of standard working solution;

[0091] (3) Setting of detection conditions;

[0092] (4) Liquid-mass spectrometry determination;

[0093] (5) Calculation of experimental results, where,

[0094] The common adulterants are norephedrine, amphetamine, amphetamine-d8, methamphetamine, methamphetamine-d8, nicotine, ephedrine, MDMA, MDMA, coffee Ketamine, Demerol, Tramadol, Methorphan, Nitrozepam, Valium, Morphine, Estazolam, Codeine, Cocaine, Alprazolam, Methadone, Cannabidiol, Thebaine, THC phenol, cannabidiol, monoacetylmorphine, fentanyl, papaverine, acetylcodeine, triazolam, heroin, narcotine.

[0095] Step (1): Take blank blood from healthy volunteers, add methcathinone to prepare blood sample 1 (methcathinone co...

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Abstract

The invention discloses a method for analyzing cathinone, methcathinone and 4-methylmethcathinone in a biological sample by liquid chromatography-mass spectrometry, which comprises the following steps: (1) preparation of sample solution; (2) preparation of standard working solution; (3) setting of detection conditions; (4) determination by liquid chromatography-mass spectrometry; and (5) experimental result calculation. The biological sample is pretreated to implement efficient and accurate detection on the cathinone, methcathinone and 4-methylmethcathinone, thereby providing technical supports for conviction and sentencing of the judicial department.

Description

technical field [0001] The invention relates to the field of drug detection in the criminal investigation field, in particular to a method for measuring cathinone, methcathinone, 4-methylmethcathinone and common adulterants in biological samples by using high performance liquid chromatography. Background technique [0002] Cathinone drugs are new drugs that have appeared in recent years and are quite harmful to the human body and society. This hazard not only affects the physiological state of the human body, but also affects people's psychological activities, and an overdose may even lead to death. Cathinone drugs mainly include cathinone, methcathinone and 4-methyl methcathinone, and their chemical structural formulas are shown in formula (1) to formula (3): [0003] [0004] In the prior art, the liquid mass spectrometry method has been applied to the detection of cathinone, methcathinone, and 4-methylmethcathinone samples, but not for actual biological samples, becau...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 常颖高利生郑珲张春水刘克林赵阳贺剑锋翟晚枫
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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