Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry

A technology of methcathinone and biological samples, which is applied in the field of drug detection in the field of criminal investigation, can solve the problems of complex biological sample system, troublesome detection work, and many interferences, and achieve high-efficiency qualitative and quantitative, good purification effect, and high detection accuracy Effect

Active Publication Date: 2015-08-12
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In the prior art, the liquid mass spectrometry method has been applied to the detection of cathinone, methcathinone, and 4-methylmethcathinone samples, but not for actual biological samples, because in actual investigation work, it is necessary to detect Most of the targets are biological

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  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry
  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry
  • Method for analyzing cathinone, methcathinone and 4-methylmethcathinone in biological sample by liquid chromatography-mass spectrometry

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Example Embodiment

[0047] Example 1

[0048] The method for analyzing cathinone, methcathinone, and 4-methylmethcathinone in urine by LC / MS includes the following steps:

[0049] (1) Preparation of sample solution;

[0050] (2) Preparation of standard working fluid;

[0051] (3) Setting of testing conditions;

[0052] (4) LC / MS determination;

[0053] (5) Calculation of experimental results, among which,

[0054] Step (1): Take three blank urine samples of healthy volunteers and add cathinone, methcathinone and 4-methylmethcathinone to prepare urine sample one (the concentration of cathinone is 500ng / mL ), urine sample two (methcathinone concentration of 500ng / mL) and urine sample three (4-methylmethcathinone concentration of 500ng / mL), respectively with water according to the volume ratio of sample to water 2:1 After proportional dilution, add protein precipitant acetonitrile, add protein precipitant until no precipitation is formed in the sample, then ultrasonically shake for 15 minutes, centrifuge at 3...

Example Embodiment

[0074] Example 2

[0075] The LC / MS method for analyzing cathinone, methcathinone, and 4-methylmethcathinone in blood includes the following steps:

[0076] (1) Preparation of sample solution;

[0077] (2) Preparation of standard working fluid;

[0078] (3) Setting of testing conditions;

[0079] (4) LC / MS determination;

[0080] (5) Calculation of experimental results, among which,

[0081] Step (1): Take three blank blood samples of healthy volunteers and add cathinone, methcathinone and 4-methylmethcathinone to prepare urine sample one (the concentration of cathinone is 500ng / mL) , Urine sample two (methcathinone concentration of 500ng / mL) and urine sample three (4-methylmethcathinone concentration of 500ng / mL), respectively diluted with water at a volume ratio of 1:1, Add the protein precipitant acetonitrile, and add the protein precipitant until no precipitation is generated in the sample. Then, after ultrasonic vibration for 15 minutes, centrifuge at 2000r / min for 20 minutes at lo...

Example Embodiment

[0087] Example 3

[0088] The method of LC / MS analysis of cathinone, methcathinone, 4-methylmethcathinone and their common adulterants in blood includes the following steps:

[0089] (1) Preparation of sample solution;

[0090] (2) Preparation of standard working fluid;

[0091] (3) Setting of testing conditions;

[0092] (4) LC / MS determination;

[0093] (5) Calculation of experimental results, among which,

[0094] The common adulterants are norephedrine, amphetamine, amphetamine-d8, methamphetamine, methamphetamine-d8, nicotine, ephedrine, methylenedioxyamphetamine, methylenedioxymethamphetamine, coffee Inine, ketamine, dolantin, tramadol, methorphan, nitroazepam, diazepam, morphine, estazolam, codeine, cocaine, alprazolam, methadone, cannabidiol, thebaine, tetrahydrocannabis Phenols, cannabidiol, monoacetylmorphine, fentanyl, papaverine, acetylcodeine, triazolam, heroin, narcotine.

[0095] Step (1): Take blank blood from healthy volunteers, add methcathinone to prepare blood sample ...

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Abstract

The invention discloses a method for analyzing cathinone, methcathinone and 4-methylmethcathinone in a biological sample by liquid chromatography-mass spectrometry, which comprises the following steps: (1) preparation of sample solution; (2) preparation of standard working solution; (3) setting of detection conditions; (4) determination by liquid chromatography-mass spectrometry; and (5) experimental result calculation. The biological sample is pretreated to implement efficient and accurate detection on the cathinone, methcathinone and 4-methylmethcathinone, thereby providing technical supports for conviction and sentencing of the judicial department.

Description

technical field [0001] The invention relates to the field of drug detection in the criminal investigation field, in particular to a method for measuring cathinone, methcathinone, 4-methylmethcathinone and common adulterants in biological samples by using high performance liquid chromatography. Background technique [0002] Cathinone drugs are new drugs that have appeared in recent years and are quite harmful to the human body and society. This hazard not only affects the physiological state of the human body, but also affects people's psychological activities, and an overdose may even lead to death. Cathinone drugs mainly include cathinone, methcathinone and 4-methyl methcathinone, and their chemical structural formulas are shown in formula (1) to formula (3): [0003] [0004] In the prior art, the liquid mass spectrometry method has been applied to the detection of cathinone, methcathinone, and 4-methylmethcathinone samples, but not for actual biological samples, becau...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 常颖高利生郑珲张春水刘克林赵阳贺剑锋翟晚枫
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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