Tumor cell growth inhibitor and its prepn process

A tumor cell growth inhibition technology, applied in the direction of antineoplastic drugs, drug combinations, etc., can solve the problems of high cost, difficult treatment methods, and low cure rate

Inactive Publication Date: 2005-03-16
钟朝晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Usually, for early-stage cancer, surgical treatment is generally adopted, supplemented by radiotherapy, chemotherapy and other means; It is difficult to have an ideal treatment method, and the mortality rate of cancer patients has always been high

Method used

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  • Tumor cell growth inhibitor and its prepn process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1), animal preparation:

[0018] Choose a Sprague Dawley (SD) female or male rat with a body weight of 250-350 grams;

[0019] (2), preparation of bone marrow cells:

[0020] SD rats were treated with CO 2 The femur and tibia were aseptically removed after asphyxiation, and the bone marrow cells were flushed out with a 10-ml syringe and an 18-gauge needle into a 10-ml petri dish containing sterile Hank's balanced salt solution. By flushing with a tapered needle, the bone marrow cell suspension was changed to its single cell suspension, and after washing with HBSS, the single cell suspension was transferred to a 20ml glass vial, and 6ml of rat lymphocyte separation solution was placed in At the bottom of the suspension, centrifuge at 1500 rpm for 30 minutes to remove red blood cells and other debris, collect the cells remaining at the interface, and wash 3 times with HBSS.

[0021] (3), cell culture:

[0022] Spread the rat bone marrow cells obtained by the above me...

Embodiment 2

[0042] The growth inhibitory effect test of the tumor cell proliferation inhibitory substance on prostate cancer cells DU-145, PC-3, LNCaP: as shown in Figure 1-3, prostate cancer cells DU-145, prostate cancer cells LNCaPU, prostate cancer cells PC-3 is effectively inhibited in the solution of the polysaccharide tumor cell growth inhibitor, and its inhibitory effect is rapidly strengthened as the concentration of the solution increases.

[0043] (1), prepare the polysaccharide tumor cell proliferation inhibitory substance as described in Example 1;

[0044] (2), preparation of tumor cell lines:

[0045] Prostate cancer cell lines DU-145, PC-3, and LNCaP were purchased from American Type Culture Collection (ATCC, ROCKVILL MARYLAND). Among them, DU-145 and LNCaP cells were respectively stored in α-MEM culture medium supplemented with 10% fetal calf serum (FCS), and PC-3 cells were preserved in DMEM culture medium supplemented with 10% fetal calf serum (FCS). All cells were sto...

Embodiment 3

[0049] Experiments on the inhibitory effect of the tumor cell proliferation inhibitory substance on different types of tumor cells:

[0050] (1), according to the above-mentioned embodiment 1, prepare the polysaccharide tumor cell proliferation inhibitory substance;

[0051] (2), preparation of tumor cell lines:

[0052] Lymphoma, bladder cancer, and epithelial cancer cell lines were preserved in RPMI-1640, DMEM, and α-MEM medium, each of which contained 10% fetal bovine serum (FBS), 100 U / ML penicillin, 100 μg / ml ML streptomycin, 25 μg / MLDE gentamicin.

[0053] (3) Experiment method:

[0054] Tumor cells in the logarithmic growth phase were treated with 2×10 4 Individual / ml concentrations were cultured for 48 hours in the presence of different dilutions of the polysaccharide tumor cell inhibitors, and the culture conditions were 37 ° C, 5% CO 2 humid environment. Add concentration of 0.5 μCi / / ml of [H 3 ] pyrantel. Cells were collected on glass fiber and filter paper, ...

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Abstract

The present invention relates to one kind of tumor cell growth inhibitor, especially one kind of water soluble and anionic radial-carrying polysaccharide, which is prepared with CD31+ marrow cell secretion of mammals and through extraction and purification of the cell culturing supernatant. It has molecular weight less than 1000 dalton and in vitro can inhibit the multiplication of tumor cells without MHC limitation and species specificity and also has one anionic radical with the complete bioactivity of the tumor cell growth inhibitor.

Description

1. Technical field: [0001] The invention relates to a tumor cell growth and proliferation inhibitory substance and a preparation method thereof, in particular to a polysaccharide tumor cell growth and proliferation inhibitory substance and a preparation method thereof, belonging to the technical field of treating tumor diseases. 2. Background technology: [0002] Cancer is the second deadliest human disease after cardiovascular disease. The treatment of cancer is a difficult problem in the world medical field. The etiology of cancer is complex, there are many types, and the mechanism is difficult to fully understand, and the comprehensive influence of individual differences, race, geography, diet, environment, genetics and other factors increases the difficulty of treatment. Usually, for early-stage cancer, surgical treatment is generally adopted, supplemented by means of radiotherapy, chemotherapy, etc.; It is difficult to have an ideal treatment method, and the mortality...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P35/00
Inventor 钟朝晖
Owner 钟朝晖
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