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Purifying method for moenomycin

A technology of moenomycin and polar solvents, which is applied in the field of moenomycin purification, can solve the problems of restricting the mass production of moenomycin, and achieve the effect of strong pertinence and high extraction yield

Inactive Publication Date: 2006-12-20
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such laboratory methods limit the production of moenomycin in large quantities

Method used

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  • Purifying method for moenomycin
  • Purifying method for moenomycin

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Experimental program
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Effect test

Embodiment 1

[0013] Fermentation product processing

[0014] Filter 10 liters of fermentation broth to obtain 1000g (wet weight) mycelium, add 2000ml 50% methanol, soak the mycelium, ultrasonically extract for 30 minutes, filter, repeat the extraction once with 50% methanol for the mycelium, and combine the extracts , concentrated under reduced pressure to remove methanol.

[0015] Add 10% sulfuric acid to the remaining aqueous solution of about 2000ml, adjust the pH value to 2.5-3.0, add an equal volume of n-butanol, extract 4 times, combine the n-butanol solution, concentrate to dryness under reduced pressure, weigh 29g, and the content of moenomycin It was 50% (as determined by HPLC method).

Embodiment 2

[0017] Separation of Moenomycin by Silica Gel Vacuum Column Chromatography

[0018] 8.0g of the above-mentioned antibiotic crude product was mixed with 20g of silica gel as a sample, put on a vacuum column (φ6.5×20) packed with 240g of silica gel in a dry method, and carried out gradient elution with n-butanol / 2M ammonia water solvent system, and the collection solvent ratio was 8 : 2 fraction 600ml, solvent ratio is 8: 3 fraction 1800ml altogether, merges fraction, concentrates under reduced pressure to obtain crude product 4.4g, and the content of moenomycin is 85% (HPLC method measures).

Embodiment 3

[0020] Magnesium silicate column chromatography decolorization

[0021] 9.0 g of the above-mentioned crude product was mixed with 18 g of magnesium silicate, wet-processed on a chromatographic column (φ2.5×55) packed with 140 g of magnesium silicate, eluted with methanol, and the eluent was 2500 ml, concentrated under reduced pressure to recover the solvent to obtain the crude product 5.2 g, the content of moenomycin is 95.2% (as determined by HPLC method).

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Abstract

The present invention relates to process of preparing moenomycin from microbial fermentation product. The preparation process includes the pre-treatment of the fermentation product, filtering fermented liquid, collecting mycelium, extracting antibiotic from the mycelium with polar solvent, and chromatographic separation to eliminate impurity, decolorize, separate and purify moenomycin. The said process has small solvent consumption, easy recovery of solvent, simple separation process, high extraction yield and high product purity up to 99 %. The product of the present invention may be used as quality control standard for the similar product, and may be used as material for medicine moenomycin bismuthate.

Description

technical field [0001] The invention relates to the research on the purification method of moenomycin, and the method of the invention can be used to prepare high-purity moenomycin from microbial fermentation products. Background technique [0002] Moenomycin (moenomycin), also known as flavomycin, bambimycin, was isolated from Streptomycesbambergiensis fermentation product by Lindetr in 1955 (K.H.Wallhausser, G.Nesemann, P.Prave, and A.Steigler, Moenomycin, a New Antibiotic Fermentation and Isolation, Farbwerke Hoechst, AG., Frankfurt am Main, Germany). Appearance is white amorphous powder, easily soluble in water, methanol, dimethylformamide, etc. [0003] Moenomycin mainly has five components, namely A, A 12 , C 1 , C 3 and C 4 , mainly A, with a content of more than 50%, and the five components all have similar antibacterial activities. Moenomycin has low toxicity and is not absorbed by the digestive tract after oral administration. After 24 hours, it is almost com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C12P19/44
Inventor 郑应华李文欣张国栋齐鹏肖进杨秀玉赵晖
Owner CHINA ANIMAL HUSBANDRY IND