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Synergetic type microbial synergistic pesticide used for preventing and controlling plant diseases, and its prepn. method

A technology for plant diseases and microorganisms, applied in botany equipment and methods, plant growth regulators, applications, etc., to achieve the effects of fast reproduction, rapid antibacterial effect, and strong colonization ability

Inactive Publication Date: 2005-01-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the applicant knows, there is no microbial compound pesticide that has a good control effect on various crop diseases including the above four diseases after compounding Bacillus subtilis and a certain pesticide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Example 1: After compounding Bacillus subtilis (Bs-916) and prochloram, spraying control of rice false smut

[0025]Compound the synergistic microbial compound pesticide of the present invention according to the following steps: transplant the Bacillus subtilis Bs-916 strain on the YPGA medium for activation; prepare the medium into a plate with prochloraz, wherein the concentration of prochloram is 5 ug / ml, 10ug / ml, 20ug / ml....... until 1000ug / ml. Transfer the activated Bs-916 strain into a 5ug / ml plate, and then transfer it into a 10ug / ml plate after it grows well, and so on until a Bs-916 resistant strain that can grow on 1000ug / ml is obtained; After activating the prochloraz-resistant Bs-916 strain, transfer it into the YPG culture medium, shake it at 28°C for 48 hours to obtain the culture medium bacterial agent, and the bacterial content is about 2.5×10 10 cfu / ml; prepare the obtained bacterial agent and prochloram at a weight ratio of 2:3 (40%:60%) to prepare t...

example 2

[0027] Example 2: After compounding Bacillus subtilis (Bs-916) and prochloram, pour water and root irrigation to prevent and cure tomato wilt

[0028] Compound the synergistic microbial compound pesticide of the present invention according to the steps similar to that of Example 1, wherein after the YPG culture solution is transferred into it, shake culture at 18°C ​​for 50 hours to obtain the culture solution bacteria agent, and the bacteria content is slightly greater than 2×10 10 cfu / ml; the obtained bacterial agent and prochloram are prepared into the synergistic microbial compound of the present invention in a weight ratio of 1: 1 (50%: 50%) and 1: 3 (25%: 75%) (Product name Jiuan).

[0029] The compound pesticide of this example was poured and root-irrigated to prevent and control tomato wilt, and it was found that it had an excellent inhibitory effect on Fusarium wilt of Solanaceae. The field test results show that the control effect is above 90%, which has excellent c...

example 3

[0030] Example 3: After compounding Bacillus subtilis (Bs-916) and prochloram, spray control of tomato leaf mold

[0031] Compound the synergistic microbial compound pesticide of the present invention according to the steps similar to that of Example 1, wherein after the YPG culture solution is moved into it, shake and cultivate for 45 hours at 32° C. to obtain the culture solution bacterial agent, and the bacterial content is about 4×10 10 cfu / ml; prepare the obtained bacterial agent and prochloram at a weight ratio of 3:1 (75%:27%) to prepare the synergistic microbial compound of the present invention.

[0032] The compound pesticide of this example is sprayed to prevent and control tomato leaf mold, and the field test results show that the control effect is more than 80%, which has an excellent control effect. Higher than similar chemicals (Table 3).

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PUM

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Abstract

A synergistic microbial agricultural chemical for preventing and controlling plant diseases is prepared through inoculating Bs-916 bacterial strains in culture medium for activating it, culturing the activated bacterial strains in the culture containing Mixianan with different concentrations to obtain resistant bacterial strains, culturing them in YPG culture liquid, and mixing with Mixianan.

Description

technical field [0001] The invention relates to a compound pesticide for preventing and controlling plant diseases, in particular to a compound pesticide of microorganisms and chemical pesticides, and at the same time, the invention also relates to a preparation method of the compound pesticide, which belongs to the field of agricultural technology. Background technique [0002] As we all know, there are many diseases on rice, fruit trees and vegetables, and the damage is serious. Among them, the occurrence and development of some diseases have seriously affected the good quality of rice, fruit trees and vegetables. As far as the applicant knows, the prevention and treatment of crop diseases mainly depends on chemical pesticides at present. Although the extensive use of chemical pesticides has played an effective role in ensuring high yields of crops and controlling the prevalence of diseases, it has also brought increasingly serious negative effects on human survival. [...

Claims

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Application Information

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IPC IPC(8): A01N63/04
Inventor 陈志谊刘永锋刘邮洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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