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Method and apparatus for electrochemical detection of biologically active substance

A biologically active substance and electrochemical technology, applied in the field of detection devices, can solve the problems of expensive detection costs of consumables, etc.

Inactive Publication Date: 2005-01-19
李伟 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of expensive consumables and high detection costs in the ELISA method, the present invention provides an electrochemical test method that can quickly and directly detect biologically active substances in samples, including:

Method used

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  • Method and apparatus for electrochemical detection of biologically active substance
  • Method and apparatus for electrochemical detection of biologically active substance
  • Method and apparatus for electrochemical detection of biologically active substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] [Example 1] In this experiment, Salmonella flagella monoclonal antibody was used to detect Salmonella.

[0048] Change the size to The platinum electrode was washed with dilute sulfuric acid and deionized water, and then dried. After mixing equal volumes of the monoclonal antibody against Salmonella and sericin solution, the surface of the platinum electrode was coated and dried naturally at room temperature to form a film. Sericin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0049] NaOH-KH with pH7.0 2 PO 4 Salmonella lO 8 Each / mL (provided by the Pasteur Institute of France) was diluted 1000, 5000, 10000 and 50000 times respectively, and an appropriate amount of silicone oil and aminoethanol were added to prepare the sample solution to be tested. Insert the working platinum electrode and a platinum electrode as a reference electrode into the socket of the interface board of ...

Embodiment 2

[0051] [Example 2] In this experiment, a mouse anti-bovine IgG antibody was used to detect IgG in bovine serum.

[0052] Change the size to The platinum electrode was washed with dilute sulfuric acid and deionized water respectively, and then dried, and the mouse anti-bovine IgG antibody (purchased from Sigama Company of the United States, diluted at 1:1000) was mixed with sericin solution in equal volumes, and the platinum electrode was coated. surface, and dry naturally at room temperature to form a film. Sericin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0053] Dilute 1:450 times with PBS mixed buffer (pH7.4 PBS199.9mL, Tween 0.1mL, bovine serum albumin BSA 0.2g, just mix), and add appropriate amount of silicone oil and aminoethanol to make the sample to be tested solution. Insert the working platinum electrode and a platinum electrode as a reference electrode into the socket of ...

Embodiment 3

[0055] [Example 3] Alpha-fetoprotein (AFP) monoclonal antibody was used to detect AFP in human serum.

[0056] Change the size to The platinum electrodes were washed with dilute sulfuric acid and deionized water, and then dried. After mixing equal volumes of alpha-fetoprotein (AFP) monoclonal antibody (purchased from Huaneng Bosai Institute of Biological Products) and sericin solution, the membrane was coated. The surface of the platinum electrode was naturally dried at room temperature to form a film. Sericin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0057] AFP-positive serum was donated by Zhenjiang Second People's Hospital. Use PBS mixed buffer (pH7.4 PBS200mL, BSA0.2g, just mix it) to dilute it to 20-200ug / L, and add appropriate amount of silicone oil and aminoethanol to make the sample solution to be tested. Insert the working platinum electrode and a platinum electrode as a r...

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Abstract

This invention provides an electrochemistry testing method for quickly and directly testing the biologically active substance in the sample, which comprises the following: to fix the relevant antibody of biologically active substance as antigen to be tested on the electrode surface through sericin to use it as working electrode; to plug the testing electrode composed by working electrode and reference electrode into reaction groove with sample to be measured and directly measure whether there is electric signal generated by combination of biologically active substance and relevant antibody specificity; then to judge the existence of biologically active substance in the testing sample. This invention also provides a testing apparatus based on this method which comprises testing electrode group, the reaction groove to contain the sample possible with biologically active substance in, fixing apparatus of the testing electrode group and measuring apparatus of electric signals.

Description

technical field [0001] The invention relates to a method for detecting biologically active substances contained in a sample, in particular to an electrochemical measurement method capable of directly detecting biologically active substances such as proteins, bacteria or viruses contained in a sample and a detection device for the detection method . Background technique [0002] The double-antibody (original) sandwich method in the commonly used enzyme-linked immunosorbent assay (ELISA) is a common method for detecting antigen (body). Enzyme-labeled antibody detects the antigen in the solution, but the consumables used in this method are expensive, the detection cost is high, and the separation and identification test cycle of biologically active substances is long. Contents of the invention [0003] In order to solve the problem of expensive consumables and high detection costs in the ELISA method, the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N33/50
Inventor 李伟陈石燕陈健俊潘宁黄文尉李幼荣
Owner 李伟
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