Method for improving desert and semi-desert soil by soil algae
A soil and desert technology, applied in the field of improvement of desert and semi-desert soil by soil algae, can solve difficult and rare problems, achieve the effect of convenient operation, increase water content, and promote stability
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[0016] Concrete steps of soil algae improving desert and semi-desert soil:
[0017] A. Separation and purification of algae species: collect microbial crusts from the semi-desert area of Siziwang Banner, Inner Mongolia, soak each gram of soil in 20mL sterile water for 12-24 hours, stir 12-24 times during the soaking process; -1 to 10 -8 Dilute, inoculate the aqueous solution onto a solid plate with a sterile dropper, add 0.04 mL of soil suspension to each plate for cultivation, and obtain a variety of algal colonies in about 21 days, mainly including Oscillatoria, Microcoleum, Nostoc, and Monofidus Algae, Chlorella, Chlorella, Protococcus; pick out the above-isolated algae species with a sterile inoculation needle or dissection needle, wash 3-4 times in sterile culture medium, and inoculate them on agar plate culture dishes Culture in; this step is repeated 8-10 times to obtain a single colony of purified culture;
[0018] B. Cultivation, screening and storage of algae spe...
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