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Mycoplasma removal composition and method for treating in-vitro cultured cells

A technology for removing compositions and mycoplasma, applied in biochemical equipment and methods, tissue culture, animal cells, etc., can solve problems such as time-consuming, pollution recurrence, etc.

Inactive Publication Date: 2021-09-10
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Time-consuming decontamination with mycoplasma and risk of contamination recurrence

Method used

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  • Mycoplasma removal composition and method for treating in-vitro cultured cells
  • Mycoplasma removal composition and method for treating in-vitro cultured cells
  • Mycoplasma removal composition and method for treating in-vitro cultured cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In the mycoplasma removal composition 1 of the present application, the final concentrations of erythromycin, chloramphenicol and tylosin are respectively 20 μM, and the final concentration of ciprofloxacin is 4 μg / ml. When used, the DMEM cell culture medium was used as the base medium, containing 10% fetal bovine serum.

Embodiment 2

[0041] In the mycoplasma removal composition 2 of the present application, the final concentrations of erythromycin, chloramphenicol and tylosin are respectively 20 μM, and the final concentrations of ciprofloxacin are 4 μg / ml. When used, MEM cell culture medium is used as the base medium, containing 10% fetal bovine serum.

Embodiment 3

[0043] In the mycoplasma removal composition 3 of the present application, the final concentrations of erythromycin, chloramphenicol and tylosin are respectively 40 μM, and the final concentrations of ciprofloxacin are 4 μg / ml. When used, the DMEM cell culture medium was used as the base medium, containing 10% fetal bovine serum.

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Abstract

The invention discloses a mycoplasma removal composition. The mycoplasma removal composition comprises erythromycin with the use final concentration of 10-40 [mu] M, chloramphenicol with the use final concentration of 10-40 [mu] M, tylosin with the use final concentration of 10-40 [mu] M and ciprofloxacin with the use final concentration of 4 [mu] g / mL. The invention is suitable for removing in-vitro cultured cells; furthermore, drug resistance of the cells and toxicity of antibiotics to the cells are easily reduced through drug combination; the original physiological activity of the cells can be easily recovered after mycoplasmas are removed; and it is guaranteed that main characteristics of the cells are not lost.

Description

technical field [0001] The application relates to the field of cell culture, in particular to a composition for removing mycoplasma and a method for treating cultured cells in vitro. Background technique [0002] Mycoplasma contamination is a major problem in cell culture, basic research, and biological research. Although mycoplasma infection may last for a long time without obvious cell damage, unlike the visibility brought by other bacterial or fungal contamination, it has a significant impact on cell endogenous activity, proliferation and other functions, which in turn causes errors in related experimental results . Mycoplasma-positive cell cultures are the primary source of infection, and positive cultures are usually discarded and replaced to prevent the spread of contamination. If cultures are considered irreplaceable or discarded, technical measures to effectively eliminate mycoplasma contamination are required. In recent years, many methods have been adopted, incl...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/09
CPCC12N5/067C12N5/0693
Inventor 高毅黎少欧楚澎彭青
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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