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Method for preparing silver sol for investigating raman spectrum

A technology of Raman spectroscopy and silver sol, which is applied in the directions of Raman scattering, color/spectral characteristic measurement, material excitation analysis, etc., and can solve the problems such as the remarkable effect of testing biomolecular Raman spectroscopy

Inactive Publication Date: 2005-08-24
NANJING NORMAL UNIVERSITY
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Problems solved by technology

It is especially effective for testing biomolecular Raman spectroscopy

Method used

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  • Method for preparing silver sol for investigating raman spectrum
  • Method for preparing silver sol for investigating raman spectrum

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Embodiment Construction

[0009] Dissolve 90 mg of silver nitrate in 500 ml of deionized water, heat to boiling, then add 10 mg of trisodium citrate with a concentration of 1% dropwise, and stir vigorously, and keep boiling for at least 2 hours after the dropwise addition. After the silver sol is naturally cooled, put it into a centrifuge test tube, and then use a centrifuge to separate the layers, remove the relatively clear sol in the upper layer, and then store it in a sealed and dark place at room temperature.

[0010] After fifteen months, take out 0.5ml of the silver sol, add 0.5ml of deionized water, then mix it with 5% DNA solution, and test to obtain a surface-enhanced Raman scattering spectrum signal. figure 1 is the laser Raman spectrum of a 5% DNA solution. figure 2 It is the surface-enhanced Raman scattering spectrum of 5% DNA solution mixed with silver sol prepared by this method. we can clearly see figure 2 The Raman peaks on the figure 1 The corresponding Raman spectrum peaks in , ...

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Abstract

The invention discloses a production technique of a silver collosol for measuring the Raman spectrum. The silver collosol produced in the technique maintains for long period, and has a stable quality which considerably enhances the Raman spectrum and effectively eliminates the fluorescence obtaining clear and stable Raman spectrum figure fast. It is effective especially on biological molecular Raman spectrum measure.

Description

Technical field [0001] The invention relates to a method for preparing and testing Raman spectrum silver sol. The prepared silver sol can be stored for a long time, and has a significant effect of surface-enhanced Raman spectrum, especially for biological macromolecules. The invention belongs to the technical field of Raman spectrum testing. Background technique [0002] At present, laser Raman technology is more and more often used to probe the molecular structure of biological macromolecules such as proteins, especially their native conformation in low concentration aqueous solution. However, most proteins will fluoresce under laser irradiation, and as long as the fluorescence appears, its intensity will always exceed the Raman signal, so that it is difficult to obtain a clearly identifiable Raman spectrum. For a long time, in order to eliminate fluorescence, the method of irradiating the test sample with a high-power laser for a long time is mainly used to degrade the fl...

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N21/65
Inventor 柯惟中周殿凤衡航吴缄中
Owner NANJING NORMAL UNIVERSITY
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