Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate

A surface-enhanced Raman and gold nanorod technology, applied in the field of chemical detection, can solve the problems of complex composition, long time consumption, influence of detection results, etc., and achieve the effect of good Raman activity

Inactive Publication Date: 2021-04-23
GUANGXI UNIV FOR NATITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection process is cumbersome, time-consuming, and low-sensitivity
Moreover, the composition of food samples is relatively complex, and there are many compone

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  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate
  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate
  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate

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Example Embodiment

[0039]The present invention is not particularly limited, and the preparative gold nano-dispersion can be satisfied with the preparation of the preparative gold nano-scattered liquid to satisfy the above parameters. In the present invention, the synthesis method of the gold nano-siphon dispersion preferably includes a seed method, a template or an electrochemical method, more preferably a seed method. In the present invention, the gold nano-dispersed liquid morphology can be controlled by the seed growth method, and is more conducive to the improvement of the Raman enhancement effect.

[0040]In the present invention, the method of synthesizing a gold nano-nano-dispersion liquid in the present invention preferably comprises the steps of:

[0041](a) mixed with tetrachloronic acid with hexadalkyltrimel bromine and sodium borohydride to obtain a mixed liquid, and the mixture was redox reaction to obtain a seed solution;

[0042](b) A mixture of hexamethyltrimel bromine, 5-bromoate, silver nitra...

Example Embodiment

[0082]Example 1

[0083](1) Provide a gold nano-dispersion, the gold nano rod is 50 nm, the concentration is 4.5 × 10-5Mol / L.

[0084]The preparation method of the gold nano-cod dispersion is as follows:

[0085]5ml 0.5 mmol / l tetrachloride (Haucl4Add to 5 ml of 0.2 M hexadalkyltrimel bromide (CTAb), stir mixed for 30 minutes. Then 0.6 ml 0.01 mol / L / L / L / L / L of sodium borohydride at 1200 rpm (NABH)4) Continue to stir for 2 minutes. With Haucl4It is mixed with CTAB, and the color of the solution gradually becomes yellow from light yellow. When Nabh4When the mixed solution is added, the color of the solution is made of yellow. The seed solution was incubated at room temperature for 30 minutes.

[0086]The growth liquid was dissolved in 5 ml of water (5-BRSA) by 180 mg of CTAb and 0.22 mg 5-bromogenic acid (5-BRSA) and then added 240 μl of 4 mM silver nitrate (AgNO3Preparation, add 5ml 1 mmol / l haucl4Previously, the mixture was mixed at room temperature for 15 minutes. Add haucl4Aft...

Example Embodiment

[0093]Example 2

[0094]The method of repeating Example 1 was different from that in the step (2) in step (2), "50 μl of solution was added from the centrifuge tube to the gold nano-dispersed liquid provided in 500 μl of step (1).

[0095]830cm in the Raman spectrum of the solution to be tested1Peak height of absorption peakkind , According to the working curve drawn according to step (2) to obtain the concentration of formaldehyde in the solution to be testedkind , Then according to formula ω = ckind × Vkind ÷ m × 100%, calculated the content ω of formaldehyde in food, and assays the solution to the solution to the test, and the calculation results are shown in Table 1.

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Abstract

The invention relates to the technical field of chemical detection, and provides a method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of a gold nanorod substrate. The method comprises the steps of reacting formaldehyde with AHMT in the presence of sodium hydroxide to generate purple 4-H-MTT, and carrying out laser Raman testing on the solution obtained by the reaction to obtain 4-H-MTT Raman spectrum. According to the linear relation between the SERS spectrum peak value and the formaldehyde concentration, the content of formaldehyde in food can be rapidly determined. Experimental results show that the formaldehyde detection limit of the method provided by the invention is as low as 1.1 nm and is equivalent to 33ng/L HCHO, and compared with the prior art, the method provided by the invention has the advantages of higher accuracy, lower detection limit and wider linear range. Moreover, in the presence of various interfering substances, the influence on the detection result is relatively small, and the selectivity on formaldehyde detection is relatively good.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for detecting formaldehyde in food based on surface-enhanced Raman spectroscopy of a gold nanorod substrate. Background technique [0002] Formaldehyde (HCHO) is a commonly used organic reagent in the fields of chemical industry, plastic industry, wood processing industry and anticorrosion. It is also a cell protoplasm poison and can damage human cell functions. Formaldehyde in food can stimulate the intestinal mucosa after entering the human body, which can cause pulmonary edema, liver and kidney congestion and perivascular edema, and even canceration. [0003] At present, the detection methods of formaldehyde mainly include acetylacetone colorimetric method, chromotropic acid titration method, liquid chromatography, fluorescence method and so on. The detection process is cumbersome, time-consuming, and low-sensitivity. Moreover, the composition of food sam...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 严军赵云霄王铭杰谭学才吴叶宇杜方凯
Owner GUANGXI UNIV FOR NATITIES
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