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Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate

A surface-enhanced Raman and gold nanorod technology, applied in the field of chemical detection, can solve the problems of complex composition, long time consumption, influence of detection results, etc., and achieve the effect of good Raman activity

Inactive Publication Date: 2021-04-23
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection process is cumbersome, time-consuming, and low-sensitivity
Moreover, the composition of food samples is relatively complex, and there are many components with similar molecular structures to formaldehyde, which will have a great impact on the detection results, resulting in low accuracy of the detection results

Method used

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  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate
  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate
  • Method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of gold nanorod substrate

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preparation example Construction

[0039] The method for preparing the gold nanorod dispersion is not particularly limited in the present invention. It is only necessary to adopt a method for preparing the gold nanorod dispersion well-known to those skilled in the art so that the prepared gold nanorod dispersion meets the above parameters. In the present invention, the synthesis method of the gold nanorod dispersion preferably includes a seed method, a template method or an electrochemical method, more preferably a seed method. In the present invention, the morphology of the gold nanorod dispersion synthesized by the seed growth method is controllable and relatively uniform, which is more conducive to improving the Raman enhancement effect.

[0040] In the present invention, the method for synthesizing the gold nanorod dispersion liquid by the seed method preferably includes the following steps:

[0041](a) tetrachloroauric acid is mixed with cetyltrimethylammonium bromide and sodium borohydride to obtain a mix...

Embodiment 1

[0083] (1) A gold nanorod dispersion is provided, the particle diameter of the gold nanorods is 50nm, and the concentration is 4.5×10 -5 mol / L.

[0084] The preparation method of gold nanorod dispersion liquid is as follows:

[0085] 5mL 0.5mmol / L tetrachloroauric acid (HAuCl 4 ) into 5 mL of 0.2M cetyltrimethylammonium bromide (CTAB), and stirred for 30 minutes. Then add 0.6mL 0.01mol / L sodium borohydride (NaBH 4 ) to continue stirring for 2 minutes. With HAuCl 4 and CTAB began to mix, and the solution color gradually changed from light yellow to yellow. When NaBH 4 When added to the mixed solution, the color of the solution changed from yellow to brown. The seed solution was incubated at room temperature for 30 minutes before use.

[0086] The growth solution consisted of 180 mg CTAB and 0.22 mg 5-bromosalicylic acid (5-BrSA) dissolved in 5 mL of water, and then 240 μL of 4 mM silver nitrate (AgNO 3 ) preparation, after adding 5mL 1mmol / L HAuCl 4 Previously, the mi...

Embodiment 2

[0094] Repeat the method of Example 1, the difference from Example 1 is that in step (2), "take 50 μL of the solution from the centrifuge tube and add it to 500 μL of the gold nanorod dispersion provided in step (1) and shake well".

[0095] In the Raman spectrum of the solution to be tested at 830cm- 1 The peak height I of the absorption peak at 样 , obtain the concentration C of formaldehyde in the sample solution to be tested according to the working curve that step (2) draws 样 , and then according to the formula ω=C 样 ×V 样 ÷m×100%, calculate the content ω of formaldehyde in the food, and measure 5 sets of parallel data of the solution to be tested. The calculation results are shown in Table 1.

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Abstract

The invention relates to the technical field of chemical detection, and provides a method for detecting formaldehyde in food based on surface enhanced Raman spectroscopy of a gold nanorod substrate. The method comprises the steps of reacting formaldehyde with AHMT in the presence of sodium hydroxide to generate purple 4-H-MTT, and carrying out laser Raman testing on the solution obtained by the reaction to obtain 4-H-MTT Raman spectrum. According to the linear relation between the SERS spectrum peak value and the formaldehyde concentration, the content of formaldehyde in food can be rapidly determined. Experimental results show that the formaldehyde detection limit of the method provided by the invention is as low as 1.1 nm and is equivalent to 33ng / L HCHO, and compared with the prior art, the method provided by the invention has the advantages of higher accuracy, lower detection limit and wider linear range. Moreover, in the presence of various interfering substances, the influence on the detection result is relatively small, and the selectivity on formaldehyde detection is relatively good.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for detecting formaldehyde in food based on surface-enhanced Raman spectroscopy of a gold nanorod substrate. Background technique [0002] Formaldehyde (HCHO) is a commonly used organic reagent in the fields of chemical industry, plastic industry, wood processing industry and anticorrosion. It is also a cell protoplasm poison and can damage human cell functions. Formaldehyde in food can stimulate the intestinal mucosa after entering the human body, which can cause pulmonary edema, liver and kidney congestion and perivascular edema, and even canceration. [0003] At present, the detection methods of formaldehyde mainly include acetylacetone colorimetric method, chromotropic acid titration method, liquid chromatography, fluorescence method and so on. The detection process is cumbersome, time-consuming, and low-sensitivity. Moreover, the composition of food sam...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 严军赵云霄王铭杰谭学才吴叶宇杜方凯
Owner GUANGXI UNIV FOR NATITIES
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