Method for quick-speed in-situ authentication of peripheral nerve tracts

Abstract

The invention relates to a method of discriminating the nature of the nerve-tract through the near infrared spectrometer and analytical method, which comprises the following steps: a) getting several nerve sport-tract and feeling-tract, sampling them by the near infrared spectrometer and getting the spectrogram; b) cluster analyzing the collecting data and establishing the model parameter used for processing of the near infrared spectrometer; c) setting the identification model of the nerve sport-tract and feeling-tract, d) scanning with different neurotransmitters in the nerve-tract in the same condition and making principal factor analysis with the wave spectrum and sport-tract wave spectrum, finding the discriminating main factor, and acquiring the nature of the nerve-tract.

Description

1. Technical field [0001] The invention relates to a method for identifying the properties of peripheral nerve bundles, in particular to a method for identifying the properties of peripheral nerve bundles by using near-infrared spectrometer technology and an analysis method thereof. 2. Background technology [0002] The identification of the nature of nerve bundles is of great significance in clinical medicine. At present, there are mainly the following ways to judge the nature of nerve bundles: [0003] 1. Anatomical method: To study the location map of the nerve bundles in the nerve trunk, many scholars have analyzed the peripheral nerves (such as the median nerve, radial nerve, ulnar and radial nerve trunk, sciatic nerve trunk, etc.) respectively, and observed the nerve trunk. The location, shape, and number of bundles of the nerve were measured, and the local location map of the nerve bundle was drawn. However, in clinical practice, the two ends of the nerve are often t...

AI Technical Summary

Problems solved by technology

However, this method needs to keep the patient awake, and is limited to fresh injuries, individual differences are large, and it is impossible to distinguish whether the muscle contraction caused by stimulation is the result of nerve conduction or current diffusion.

Therefore, the clinical application value is not large

[0005] 3. Radiobiochemical method: use radioactive isotope to label the substrate of the enzyme, and then measure the activity of the enzyme according to the radioactive product produced by the enzyme reaction, and speculate on the nature of the nerve bundle. However, this method involves isotope technology, high equipment requirements, and radiation. The substance is harmful to both the patient and the operator, so it is not very practical

However, there are problems with this method: (1) the staining time is long (about 50 minutes), and the process is complicated; (2) two nerve segments need to be cut out, and in situ staining cannot be performed

However, the problem with this method is that the sensor needle must be replaced after each nerve bundle is detected, and cannot be reused. It still takes a few minutes to detect a nerve bundle, which is a bit too long

The problem is that the activity of the enzyme and the detection process are greatly disturbed, and there is also a problem of disinfection and storage, which cannot meet the clinical needs