Nanometer grainy and mucosal immunity of antiatherosclerosis nucleic vaccine

An atherosclerotic nucleic acid, mucosal immunity technology, applied in the direction of antibody medical ingredients, medical preparations containing active ingredients, allergic diseases, etc., can solve problems such as hindering the development of such vectors

Inactive Publication Date: 2005-10-12
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gene carriers are divided into two categories: viruses and non-viruses. Viruses, as a gene carrier with a high transfection rate, have been studied for many years, but their safety problems such as immunogenicity and tumorigenicity exposed in clinical practice hinder The further development of such vectors, in recent years, people are committed to finding safe and effective non-viral vectors

Method used

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  • Nanometer grainy and mucosal immunity of antiatherosclerosis nucleic vaccine
  • Nanometer grainy and mucosal immunity of antiatherosclerosis nucleic vaccine
  • Nanometer grainy and mucosal immunity of antiatherosclerosis nucleic vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An appropriate amount of chitosan was dissolved in 5 mM acetic acid-sodium acetate buffer solution (pH5.5) to prepare a solution with a concentration of 2 mg / ml. An appropriate amount of anti-atherosclerosis nucleic acid vaccine was dissolved in 15mM sodium sulfate solution to prepare a solution with a concentration of 500 μg / ml. Heat the above two solutions in a water bath at 55°C for 5 minutes, spin up 1 volume of the nucleic acid vaccine solution with a vortex shaker, quickly pour 0.5 volume of chitosan solution into the nucleic acid vaccine solution, and vortex for 20 seconds. Stand still at room temperature for more than 1 hour to obtain the colloidal solution of anti-atherosclerosis nucleic acid vaccine / chitosan nanoparticles.

Embodiment 2

[0021] An appropriate amount of chitosan was dissolved in 5 mM acetic acid-sodium acetate buffer solution (pH 5.5) to prepare a solution with a concentration of 300 μg / ml. An appropriate amount of anti-atherosclerosis nucleic acid vaccine was dissolved in 15mM sodium sulfate solution to prepare a solution with a concentration of 100 μg / ml. The above two solutions were heated in a water bath at 55° C. for 5 minutes, mixed rapidly with equal volumes, vortexed for 30 seconds, and left at room temperature for 1 hour to obtain a colloidal solution of anti-atherosclerotic nucleic acid vaccine / chitosan nanoparticles.

Embodiment 3

[0023] An appropriate amount of chitosan was dissolved in 5 mM acetic acid-sodium acetate buffer solution (pH5.5) to prepare a solution with a concentration of 2 mg / ml. An appropriate amount of anti-atherosclerosis nucleic acid vaccine was dissolved in 6.25mM sodium sulfate solution to prepare a solution with a concentration of 800 μg / ml. The above two solutions were heated in a water bath at 55° C. for 5 minutes, mixed rapidly with equal volumes, vortexed for 30 seconds, and left at room temperature for 1 hour to obtain a colloidal solution of anti-atherosclerotic nucleic acid vaccine / chitosan nanoparticles.

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Abstract

A nanoparticle of the anti-atherosclerosis nucleic acid vaccine is rpepared from chitosan solution and the anti-atherosclerosis nucleic acid vaccine plasmid DNA through copolymerization, and features that it takes its immune effect via nasal mucosa. It can induce the generation of the specific antibody to cholesteryl ester transfer protein with high level of said antibody.

Description

technical field [0001] In the field of medicine, the anti-atherosclerosis nucleic acid vaccine / chitosan nanoparticle provided by the present invention can be conveniently applied to mucosal immunity, and the level of specific antibodies induced by the body is better than that of intramuscular injection of simple nucleic acid vaccine. Background technique [0002] Atherosclerosis (AS) is a common disease that seriously endangers human health, and its incidence is increasing significantly in our country. The occurrence of atherosclerosis is the result of multiple factors, and its etiology has not been fully elucidated. It is generally believed that cholesteryl ester transfer protein (CETP) is closely related to the occurrence and development of atherosclerosis. Nucleic acid vaccine pCR3.1-X constructed by our laboratory 8 -HBc-CETPC, through intramuscular injection, can effectively produce specific anti-CETP antibody, and can effectively prevent the occurrence of atheroscler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K39/00A61K48/00A61P9/12A61P37/02
Inventor 宗莉顾凯龙军王学军陈伶俐曹荣月吴洁刘景晶
Owner CHINA PHARM UNIV
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