Recombinant plasmids containing B cell activation factor gene promoters with different lengths and their preparation method and application

Abstract

The invention relate to the field of biomedical engineer technical. At present, the study that the structure of promoter, function analysis and transcriptional controlling of B-cell activating factor are vacant, the invention provide five kinds of different length recombinant plasmid of B-cell activating factor gene promoter, the recombination plasmid is composed by the sequence -1349~-1099bp, -1349~-893bp, -1349~-743bp, -1349~-431bp, -1349~-329bp upstream promoter of human BAFF gene and the vector not contain the promoter, but contain the report gene of chloramphenicol acetyltransferase CAT. The invention also provides the preparative method and usage of the recombination plasmid. The invention constructing different length recombination plasmid of BAFF gene promoter is accurate and reliable, establishing a base for the advanced study in transcriptional control of human BAFF gene activation and the report gene live determining is easier than comparing recombination promoter transcription, and can quantitate, the method is more convenient to select the drug that rivalry or treat SLE in transcription level, the method also provide new target for exploring prevention and cure SLE.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to five recombinant plasmids containing different lengths of B cell activation factor (BAFF) gene promoters and their preparation methods and uses. Background technique [0002] Systemic lupus erythematosus (SLE) is a common and frequently-occurring disease, mainly manifested in the excessive proliferation and activation of B cells, the production of various autoantibodies such as anti-double-stranded DNA antibodies, and through immune complexes and other ways, causing Almost every system and organ in the body is affected. The disease mostly occurs in young and middle-aged women aged 20 to 50. According to reports, the prevalence of SLE in my country is close to 1‰, and the total number of patients in the country is as high as 1 million. If the diagnosis and treatment are not timely, 70-80% of SLE patients will suffer from renal failure, seriously affecting the quali...

AI Technical Summary

Problems solved by technology

[0005] However, in recent years, the research progress and trend of BAFF at home and abroad have mainly focused on exploring the relationship between BAFF and SLE immunopathology at the protein and nucleic acid levels, and there is obviously a lack of research on the structure, function analysis and transcriptional regulation of the BAFF gene promoter. ...

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