The invention relates to a method for detecting secretory cells of a peripheral blood hepatitis B surface antibody. According to the method disclosed by the invention, human Peripheral Blood Mononuclear Cells (PBMC) are stimulated and activated in vitro, and a pretreated Polyvinylidene Fluoride (PVDF) plate is used for detecting anti-HBs secretory cells in the PBMCs, to detect that a million of PBMCs contain one anti-HBs secretory cell. The method overcomes the detection difficulties in peripheral blood anti-HBs secretory cells, such as small quantity of the peripheral blood anti-HBs secretory cells, low detection rate, low experiment repetitiveness, complex operation, long time consumption and high difficulty, solves the problems that much peripheral blood needs to be adopted and in-vitro B cell activation methods such as CPG, CD40L and pokeweed mitogen are low in activation efficiency, and overcomes the defects of reduction of the detection rate of the anti-HBs secretory cells and the like. According to the method, R848, HBsAg and IL-2 are used for stimulating and activating peripheral blood B cells, and the HBsAg pre-coated PVDF plate is used for capturing the peripheral blood anti-HBs secretory cells, so that the blood amount for detection is reduced, and the detection sensitivity is improved.