Recombinant antigen protein with function of easily activating B cells and preparation method thereof
A technology for recombining antigens and B cells, applied in the field of immunology, can solve problems such as uneven assembly, increased activation of B cells, epitope blocking, etc., and achieve the effects of reducing the vaccination dose, prolonging the duration of antibodies, and increasing the molecular weight of proteins
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Embodiment 1
[0141] Embodiment 1 classical swine fever virus recombinant antigen
[0142] This example provides a CSFV recombinant antigen that can easily activate B cells and a preparation method thereof.
[0143] 1. Determine the target protein:
[0144] Classical swine fever (CSF) is a highly contagious, acute and fatal infectious disease caused by classical swine fever virus (CSFV). The CSFV genome is a single-stranded positive-strand RNA, which is a spherical enveloped virus. The structure of CSFV was analyzed by literature search and bioinformatics prediction ( Figure 4 ) found that the four major structural proteins of CSFV are C, Erns (gp44 / 48), E1 (gp33), and E2 (gp55). Among them, the molecular weight of structural protein E2 is about 55kDa, which is a glycosylated envelope protein, mainly located on the surface of virus particles, and plays an important role in the process of virus invasion into cells; at the same time, E2 protein is also the main protective antigen of CSFV,...
Embodiment 2
[0178] Example 2 Newcastle disease virus recombinant antigen
[0179] This example provides a recombinant Newcastle disease virus antigen that can easily activate B cells and a preparation method thereof.
[0180] 1. Determine the target protein:
[0181] Newcastle disease virus (NDV) is an approximately spherical particle with an envelope, and often has an irregular shape due to the damage of the envelope, and filaments of different lengths with a cross-sectional diameter of about 100 nm can be seen. Through literature search and bioinformatics prediction analysis of NDV virus structure ( Figure 15). The surface of the viral envelope is covered with 8nm-long filaments, and the inside of the virion is a coiled nucleocapsid with a diameter of about 17nm. The six specific structural proteins of NDV are L protein (large protein, large protein), NP (nucleuo protein, nucleoprotein), P protein (phosphor protein, phosphoprotein), M protein (matrix protein, matrix protein), F prot...
Embodiment 3
[0222] Example 3 Novel Coronavirus Recombinant Antigen
[0223] This example provides a novel coronavirus recombinant antigen that is easy to activate B cells and a preparation method thereof.
[0224] 1. Determine the target protein:
[0225] Analysis of SARS-CoV-2 virus structure through literature search and bioinformatics prediction ( Figure 28 ), the four main structural proteins of SARS-CoV-2 are spike protein (Spike protein, S), envelope protein (Envelop protein, E), matrix protein (Membrane protein, M) and nucleocapsid protein (Nucleocapsid protein , N). Among them, the S protein is an important class of glycoproteins on the surface of coronaviruses, including key epitopes of coronaviruses, and is the main target for pathogen detection and vaccine research. Therefore, S protein was chosen as the target protein.
[0226] 2. Structural analysis of target protein and identification of key epitopes
[0227] 1) Target protein secondary structure prediction
[0228] T...
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