Process for preparing oligomeric peptide capable of inhibiting angiotonase activity

An angiotensinase and oligopeptide technology, applied in the field of oligopeptides, can solve the problems of poor inhibitory effect, high cost, and the preparation of ACE inhibitory active peptides from eggplant ginseng collagen, etc., and achieves high activity and good product activity. inhibitory effect
CN1858230AInactive Publication Date: 2006-11-08OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2006-11-08
Estimated Expiration
Not applicable · inactive patent
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Abstract

The preparation process of oligomeric peptide capable of inhibiting angiotonase activity includes the following steps: pulping Acaudia molpadioides with water, centrifuging to obtain precipitate, washing with alkali solution, water washing the precipitate to neutrality, water extracting while heating, centrifuging to obtain hot water extracted collagen solution, freeze drying to obtain Acaudia molpadioides collagen, dissolving in buffering phosphoric acid-citric acid solution, adding bromelin through stirring at constant temperature to enzymolyze, deactivating enzyme, cooling, ultrafitering with ultrafiltering membrane, regulating pH to 7-8, adding Alcalase enzyme through stirring at constant temperature to enzymolyze, deactivating enzyme, cooling, ultrafiltering, adding type I collagenase to enzymolyze at constant temperature, deactivating enzyme, cooling, ultrafiltering to obtain hydrolysate, chromatographic separating, and freeze drying.
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Description

technical field

[0001] The invention relates to a method for preparing oligopeptides capable of inhibiting angiotensinase activity by directional enzymatically hydrolyzing eggplant ginseng collagen with compound enzymes Background technique

[0002] Essential hypertension is caused by a variety of factors, including heredity, eating habits, and mental state. Studies have shown that whether blood pressure is normal or not is closely related to the renin-angiotensin system and the kallikrein-kinin system, and angiotensinase (ACE) plays an important role in the above two systems. In the renin-angiotensin system, ACE hydrolyzes non-pressor-active angiotensin I to produce pressor-active angiotensin II; while in the kallikrein-kinin system, ACE can degrade the The antihypertensive bradykinin loses its antihypertensive activity. ACE participates in the regulation of blood pressure in the body through the above two ways, which can cause the increase of blood pressure and cause hyp...

Claims

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