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Process for preparing oligomeric peptide capable of inhibiting angiotonase activity

An angiotensinase and oligopeptide technology, applied in the field of oligopeptides, can solve the problems of poor inhibitory effect, high cost, and the preparation of ACE inhibitory active peptides from eggplant ginseng collagen, etc., and achieves high activity and good product activity. inhibitory effect

Inactive Publication Date: 2006-11-08
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

Food-derived ACE-inhibiting active peptides have high safety, mild, specific and long-lasting antihypertensive effects, and no side effects, but their disadvantages are high cost and poor inhibitory effect on ACE
[0003] At present, there is no report on the preparation of ACE inhibitory active peptides from eggplant ginseng collagen

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0007] Add 1 kg of eggplant ginseng body wall to 2.5 kg of distilled water for beating, centrifuge, stir the obtained residue with 8 kg of NaOH aqueous solution overnight, centrifuge, repeat washing with NaOH aqueous solution three times, remove the supernatant, rinse the precipitate with distilled water until neutral, add distilled water Heating at 45°C with stirring and extraction for 8 hours, centrifugation to obtain collagen aqueous solution, and freeze-drying. Gained eggplant ginseng collagen is dissolved in phosphoric acid-citric acid buffer solution (the molar concentration of this buffer solution is 0.02M, pH4.5) and is made into 0.5~1.5% (weight percent concentration, the same below), preferably 1% The solution is 4000-5000U / g, preferably 4500U / g collagen plus bromelain, stirred at constant temperature, hydrolyzed at 40-50°C, preferably 45°C for 3-5 hours, preferably 4 hours, 95-100°C Inactivate the enzyme, cool, and ultrafilter with a 10KDa ultrafiltration membrane; ...

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Abstract

The preparation process of oligomeric peptide capable of inhibiting angiotonase activity includes the following steps: pulping Acaudia molpadioides with water, centrifuging to obtain precipitate, washing with alkali solution, water washing the precipitate to neutrality, water extracting while heating, centrifuging to obtain hot water extracted collagen solution, freeze drying to obtain Acaudia molpadioides collagen, dissolving in buffering phosphoric acid-citric acid solution, adding bromelin through stirring at constant temperature to enzymolyze, deactivating enzyme, cooling, ultrafitering with ultrafiltering membrane, regulating pH to 7-8, adding Alcalase enzyme through stirring at constant temperature to enzymolyze, deactivating enzyme, cooling, ultrafiltering, adding type I collagenase to enzymolyze at constant temperature, deactivating enzyme, cooling, ultrafiltering to obtain hydrolysate, chromatographic separating, and freeze drying.

Description

technical field [0001] The invention relates to a method for preparing oligopeptides capable of inhibiting angiotensinase activity by directional enzymatically hydrolyzing eggplant ginseng collagen with compound enzymes Background technique [0002] Essential hypertension is caused by a variety of factors, including heredity, eating habits, and mental state. Studies have shown that whether blood pressure is normal or not is closely related to the renin-angiotensin system and the kallikrein-kinin system, and angiotensinase (ACE) plays an important role in the above two systems. In the renin-angiotensin system, ACE hydrolyzes non-pressor-active angiotensin I to produce pressor-active angiotensin II; while in the kallikrein-kinin system, ACE can degrade the The antihypertensive bradykinin loses its antihypertensive activity. ACE participates in the regulation of blood pressure in the body through the above two ways, which can cause the increase of blood pressure and cause hyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02A61K36/87A61P9/12
Inventor 曾名湧赵元晖刘尊英董士远李八方
Owner OCEAN UNIV OF CHINA
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