Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SCAR label of Chinese cabbage orange leaf-head and its use in auxiliary selective breeding

A technology of Chinese cabbage and orange, which is applied to the SCAR marker of Chinese cabbage orange leaf bulbs, and the application field of assisted selection breeding, which can solve the problems of delaying the breeding process, time-consuming and laborious, etc., and achieve the effect of accurate selection of molecular breeding

Inactive Publication Date: 2007-04-11
NORTHWEST A & F UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the traditional color phenotype selection of leaf bulbs should be carried out at the end of heading, the field observation of bulb cutting is used, which is time-consuming and laborious to operate, which greatly delays the breeding process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SCAR label of Chinese cabbage orange leaf-head and its use in auxiliary selective breeding
  • SCAR label of Chinese cabbage orange leaf-head and its use in auxiliary selective breeding
  • SCAR label of Chinese cabbage orange leaf-head and its use in auxiliary selective breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] The present invention has prepared 4 sets of F 1 , F 2 , BC1 materials are research materials, respectively as follows:

[0063] (1) Female parent: Zaohuangbai-4, male parent: S941-6, backcross parent: S941-6;

[0064] (2) Female parent: S941-1, male parent 01S160-1, backcross parent: S941-6;

[0065] (3) Female parent: S941-7, male parent 77M-5, backcross parent: S941-6;

[0066] (4) Female parent: S941-2, male parent 01Sb5-1, backcross parent: S941-6;

[0067] The study used RAPD technology to screen specific primers related to orange leaf bulbs, further cloned and sequenced the specific fragments, and converted them into SCAR markers. Through the segregation performance of F2 generation, the linkage distance was calculated, and SCAR markers were applied to molecular breeding.

[0068] Firstly, the PCR reaction system was optimized, followed by the screening of specific primers, the cloning and sequencing of specific bands, the design of SCAR primers, the verifica...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses one kind of SCAR label of orange Chinese cabbage leaf bulb and its application in auxiliary selective breeding. The auxiliary selective breeding method includes adopting the genome of color Chinese cabbage, common white Chinese cabbage and their second filial generation segregating colony as the template, RAPD analysis and repeated experiments to screen out RAPD marker linked with the orange leaf bulb of color Chinese cabbage and convert into one specific SCAR marker successfully. It is found through linked analysis that the linking distance between the marker and the orange leaf bulb is 3.7cm. The SCAR marker may be used in molecular auxiliary selective breeding of orange leaf bulb character of Chinese cabbage.

Description

technical field [0001] The invention belongs to the field of vegetable variety breeding and biotechnology in agriculture, and relates to a method for variety breeding and molecular marker-assisted selection breeding of Chinese cabbage, in particular to a SCAR marker of orange leaf bulbs of Chinese cabbage and its application for auxiliary selection breeding. Background technique [0002] Chinese cabbage originated in my country, and its cultivation area and output rank first among vegetables. The quality and nutrition of Chinese cabbage are closely related to people's health. With the improvement of people's living standards, the requirements for the quality of Chinese cabbage are getting higher and higher. The colorful Chinese cabbage that has appeared at present has good-looking colors, high nutritional value, and is very popular for its advantages such as processing, pickling, and no discoloration. [0003] The Vegetable Science Department of the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 张鲁刚王绮张战风惠麦侠张明科
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com