Extract of soil actinomycetes fermenting liquid and its prepn and application

A technology of soil actinomycetes and fermentation broth, applied in the application field of pesticides, can solve the problems of drug resistance and other problems, and achieve the effects of high economic and social benefits, simple fermentation process, and simple extraction process

Inactive Publication Date: 2007-06-06
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of these agents has resul

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Extract of the present invention - the preparation method of brown oily thing:

[0031] (1) Seed culture of soil actinomycetes GDPPRA 3704:

[0032] Gao's No. 1 medium, the medium composition is: KNO 3 1g, NaCl 0.5g, MgSO 4 0.5g, K 2 HPO 4 0.5g, FeSO 4 0.01g, 20g of soluble starch, 20g of agar, and 1000ml of water were used to make an eggplant bottle slant, and the bacterial strain spores were applied to the slant, and cultivated at 28°C for 10 days.

[0033] (2) Liquid fermentation culture of soil actinomycetes GDPPRA 3704:

[0034] Use a 30L fermenter for liquid fermentation culture. The fermentation medium is: 20g glucose, 10g starch, 15g bean cake powder, 15g corn steep liquor, 5g sodium chloride, 3g calcium carbonate, and 1000ml water; make spores from mature strains in the eggplant bottle slope The suspension was put into a fermenter, the inoculum size was 10%, the fermentation temperature was 30° C., the ventilation rate (V / Vmin) was 1:0.8, and the ferm...

Embodiment 2

[0040] Extract of the present invention - the preparation method of brown oily thing:

[0041] (1) Seed culture of soil actinomycetes GDPPRA 3704:

[0042] Gao's No. 1 medium, the medium composition is: KNO 3 1g, NaCl 0.5g, MgSO 4 0.5g, K 2 HPO 4 0.5g, FeSO 40.01g, 20g soluble starch, 20g agar, 1000ml water, make a test tube slant, inoculate the spores of the strain to smear the slant, and cultivate at 28°C for 8 days.

[0043] (2) Liquid fermentation culture of soil actinomycetes GDPPRA 3704:

[0044] Use a 30L fermenter for liquid fermentation culture. The fermentation medium is: 30g of glucose, 15g of starch, 25g of bean cake powder, 10g of corn steep liquor, 8g of sodium chloride, 8g of calcium carbonate, and 1000ml of water; the mature strains in the inclined plane of the test tube are made into spore suspension The solution was introduced into a fermenter, the inoculum size was 16%, the fermentation temperature was 26° C., the ventilation rate (V / Vmin) was 1:1.2...

Embodiment 3

[0049] Example 3 The extract of the present invention-brown oily matter kills diamondback moth and Chinese bream activity experiment

[0050] A. Anti-Plutella xylostella test

[0051] The brown oil obtained in Example 1 was diluted in serial concentration, and then the cabbage leaves were soaked in the solution for 30 seconds, taken out, and after drying naturally, the 2nd age Plutella xylostella larvae artificially cultured were received on the leaves treated with the medicament, Cultured at 25°C, the mortality of Plutella xylostella was investigated every 24 hours, acetone was used as the blank control, and each treatment was replicated three times. The experimental results are shown in Table 1. The drug concentration is 175 μg / ml, 72 hours can kill diamondback moth 100%; the concentration is 350 μg / ml, 48 hours can kill diamondback moth 100%.

[0052] Table 1

[0053] Concentration (μg / ml)

[0054] B. Tests against fish parasites, Chine...

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Abstract

The present invention provides extract of soil actinoycetes GDPPRA 3704 fermenting liquid and its preparation process and application in preparing pesticide for killing cabbage moth, fish parasite, etc. The extract is obtained through seed culture of soil actinoycetes GDPPRA 3704, liquid fermentation culture of soil actinoycetes GDPPRA 3704, leaching effective matter, and separating effective matter. The present invention has simple fermentation process and extraction process, and high effective matter recovering rate. The extract in relatively low concentration has effect of killing cabbage moth, fish parasite, etc. and is hopeful in preparing biological pesticide with less environment influence.

Description

technical field [0001] The invention relates to an extract of soil actinomycete fermented liquid isolated from the Nalati region of Xinjiang and a preparation method thereof, as well as the application of the extract in preparing pesticides against diamondback moth and fish parasite Sinensis sinensis. Background technique [0002] Diamondback moth (Plutella xylostella Linnaeus) belongs to Lepidoptera Plutellaidae and is the main pest of cruciferous vegetables worldwide. It has many generations (generally 8 to 12 generations in subtropical areas and more than 20 generations in tropical areas) and has a high reproductive coefficient. On average, each female adult lays more than 170 eggs, and the number of insect populations increases rapidly between generations, and the overlapping phenomenon is serious. In the whole world, the economic loss caused by diamondback moth is very serious. In the United States, the annual loss caused by insect pests reaches...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/02C12R1/04
Inventor 沈会芳林壁润谢双大潘群英姚新生张广文
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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