Anti-HTLV-1 drug and therapeutic agent for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP)
a therapy agent and anti-htlv-1 technology, applied in the field of anti-htlv1 drugs, can solve the problems of further problematic treatment methods
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
[0078]As mentioned in Experimental Example 1 described below, as a result of microarray analysis and pathway analysis using CD4+ T cells from HTLV-1-associated myelopathy (HAM) patients, pathways including the ABL1 gene were identified as pathways including a gene with a significantly increased expression level in CD4+ T cells from HAM patients. In this Example, imatinib (Glivec / Gleevec) and nilotinib (Tasigna), which are inhibitors of tyrosine kinase encoded by the ABL1 gene, were used to examine the influence of these drugs on CD4+ T cells from HAM patients.
[0079][1] Measurement of the cell concentration of human PBMCs (assay sensitivity determination) by CellTiter-Fluor Cell Viability Assay (G6080, Promega Corporation) (# TB (Technical Bulletin) 371: Instructions for use of Products G6080. G6081, and G6082 (Promega)) (This kit is to quantify the intensity of fluorescence derived from cleavage of a GF-AFC substrate by live cell protease, which is proportional to the number of live...
experimental example 1
[0111]In Experimental Example 1, pathways including genes significantly and increasingly expressed in CD4+ T cells from HTLV-1-associated myelopathy patients were identified by microarray analysis and pathway analysis using CD4+ T cells from HTLV-1-associated myelopathy patients. FIG. 4 depicts the flowchart of the analysis method in this experiment.
[0112]Based on clinical diagnosis according to the WHO diagnostic criteria, 4 cases of HTLV-1-associated myelopathy patients, 4 cases of asymptomatic HTLV-1 carriers (ACs), and 4 cases of HTLV-1 negative healthy controls (NCs) were randomly selected (Table 2). In consideration of ethical issues, blood collection and specimen preservation were carried out with sufficient explanation and written consent. In this study, patients and specimens were anonymized in an unlinkable manner, and specimens obtained under approval from the Ethics Committee of Kagoshima University were used.
[0113]
TABLE 2HAMACNCNumber of specimens444Gender (Male:Female)...
example 2
[0152]In this Example, a novel quantitative determination method, PMA (propidium monoazide)-HTLV-1 viability PCR, by which the proviral load (PVL) of HTLV-1 only in live cells can be quantitatively determined, was developed. This technique was used to examine whether ABL1 inhibitors have an effect of reducing the proviral load of HTLV-1 in living cells. In the usual PVL measurement method, PVL of dead cells and PVL of living cells cannot be distinguished from each other for measurement, meaning that PVL is collectively measured for both cells.
[0153]PMA-HTLV-1 viability PCR, which is a novel quantitative determination method, will be described in detail in Experimental Example 2 below. The method is based on conventionally known PMA viability PCR (Nocker A. et al. J Microbiol Methods 2006. 67: 310-320).
(1) Assay of ABL1 Inhibitors by PMA-HTLV-1 Viability PCR
(1-1) Preparation of Experimentation
(1-1-1) Subjects / Cells
[0154]PBMCs from 16 HAM cases frozen and preserved in liquid nitrogen ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| volume | aaaaa | aaaaa |
| volume | aaaaa | aaaaa |
| volume | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More