Methods for enhanced epithelial permeation of y2 receptor-binding peptides for treating and preventing obesity

Abstract

A method for treating obesity, inducing weight-loss, or inducing satiety in a mammal comprising administering intranasally to the mammal a therapeutically effective amount of a pharmaceutical composition comprising PYY(3-36), a phosphatidylcholine or diglyceride, and a cyclodextrin, wherein the phosphatidylcholine or diglyceride and the cyclodextrin are present in an amount sufficient to enhance epithelial permeation.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a divisional claiming the benefit under 35 U.S.C. § 120 of U.S. patent application Ser. No. 10 / 869,649, filed Jun. 16, 2004, which is a continuation-in-part application and claims priority under 35 U.S.C. § 120 of co-pending, U.S. patent application Ser. No. 10 / 745,069 filed Dec. 23, 2003, which is a continuation-in-part of U.S. patent application Ser. No. 10 / 322,266, filed Dec. 17, 2002, and claims priority under 35 U.S.C. § 119(e) of U.S. Provisional Application No. 60 / 493,226, filed Aug. 7, 2003, U.S. Provisional Application No. 60 / 501,170, filed Sep. 8, 2003, U.S. Provisional Application No. 60 / 510,785, filed Oct. 10, 2003, U.S. Provisional Application No. 60 / 517,290, filed Nov. 4, 2003; U.S. Provisional Application No. 60 / 518,812, filed on Nov. 10, 2003; the entire contents of these applications are incorporated herein by reference.BACKGROUND OF THE INVENTION [0002] The teachings of all the references cited in t...

AI Technical Summary

Benefits of technology

[0012] The present invention fulfills the foregoing needs and satisfies additional objects and advantages by providing novel, effective methods and compositions for delivery, especially mucosal, most especially intranasal, delivery of a Y2 receptor-binding peptide such as PYY, Pancreatic Peptide (PP) and NPY, to treat obesity, induce satiety in an individual and to promote weight-loss in an individual and prevent or cure diabetes. In certain aspects of the invention, the Y2 receptor-binding peptide is delivered in formulations to the intranasal mucosa so as to be able to increase the concentration of the Y2 receptor-binding peptide by at least 5 pmol, preferably by at least 10 pmol, in the blood plasma of a mammal when a dose of the formulations of the Y2 receptor agonist is administered intranasally. Furthermore preferred formulations would be able to raise the concentration of the Y2 receptor-binding peptide in the plasma of a mammal by 10 pmol, preferably 20 pmol, when the Y2 receptor-binding peptide is administered intranasally. When 150 μg is administered intranasally the preferred formulation would be able to raise the concentration of the Y2 receptor agonist in the plasma of the mammal by at least 40 pmol per liter of plasma. When 200 μg of the Y2 receptor-binding peptide is administered intranasally, the formulations of the present invention induce at least 80 pmol, per liter of plasma increase of the Y2 receptor-binding peptide. In preferred embodiments, the elevated concentrations of the Y2-receptor-binding peptide remains elevated in the plasma of the mammal for at least 30 minutes, preferably at least 60 minutes following a single intranasal dose of the Y2 receptor-binding peptide.

Problems solved by technology

Obesity and its associated disorders are common and very serious public health problems in the United States and throughout the world.

It reduces life-span and carries a serious risk of co-morbidities above, as well disorders such as infections, varicose veins, acanthosis nigricans, eczema, exercise intolerance, insulin resistance, hypertension hypercholesterolemia, cholelithiasis, orthopedic injury, and thromboembolic disease.

However, for the treatment of obesity and related diseases, including diabetes, the mode of administration has been limited to intravenous IV infusion with no effective formulations optimized for alternative administration of PYY3-36.

However, the physical forces associated with droplet formation often destroy or denature proteins and peptides.

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