Methods for determining cellular response to stimuli

a technology of cellular response and stimuli, applied in the direction of material analysis, instruments, biological material analysis, etc., can solve the problems of limited development of new agents that mimic or block the basic tastes, high calories, and many taste-mimicking or taste-blocking agents developed to-date are not suitable food additives

Inactive Publication Date: 2008-02-28
PAUL KIM CHIN HONG +8
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many taste-mimicking or taste-blocking agents developed to-date are not suitable as food additives, however, because they are not economical, are high in calories, or are carcinogenic.
Development of new agents that mimic or block the basic tastes has been limited by a lack of knowledge of the taste cell biology involved in the transduction of taste modalities.

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  • Methods for determining cellular response to stimuli
  • Methods for determining cellular response to stimuli
  • Methods for determining cellular response to stimuli

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example 1

Multiphoton Fluorescence Lifetime Imaging System Characterization: Response to Calcium Green Dextran Solutions of Known Free Calcium Concentration

[0099] The present inventors made use of a multiphoton lifetime fluorescence imaging microscopic (MP-FLIM) system capable imaging calcium dynamics within taste cells in intact, live porcine taste tissue. The approach made use of a calcium sensitive fluorescent dye (calcium green-1 dextran MW=3000), where the dye emission properties (fluorescence lifetime and intensity) were perturbed by intracellular calcium concentration changes in the nanomolar to millimolar range. Multiphoton-excited fluorescence, in this case, made use of a pulsed near-infrared laser to excite fluorescence from a visible fluorophore via a two or three photon process. This non-linear optical process allowed for deep tissue imaging of fluorophore emission with minimal cell damage from the near-infrared laser due to lack of absorption outside of the focal plane and absen...

example 2

Quantitative Algorithm Development and Estimation of System Optical Performance

[0109] There are a number of tasks involved to ensure precise and robust measurement of changes that occur in the image data obtained from the MP-FLIM system. First, an attempt should be made to mitigate artifacts induced by the optical path and detector on the “true” image data. In a spatially invariant system (i.e., all the pixels in the field of view experience the same optical and detector effects), such artifacts can be classified as either resolution loss (blurring) or recorded pixel intensity errors (noise). Second, because the image data are recorded in a flow cell, the image features may move over time, the process of image registration compensates for such movement by re-aligning the images in a given time-series with respect to a reference state (usually the first image in the series). Proper image registration is necessary when using region-of-interest (ROI) methods to quantify intensity chan...

example 3

Development of Tissue Preparation Protocols

[0119] The utilization of porcine taste tissue in taste research is a new approach in taste research at the cellular level. Several researchers have performed behavioral studies with pigs (Kare, et. al, 1965) and one has performed electrophysiological recordings from nerve fibers and whole nerves innervating the peripheral taste system of pigs exposed to various stimuli (Danilova, 1999). These works provide a basis for the similarity between human and porcine taste responses, in bulk, but provide no knowledge regarding the anatomy and physiology of the porcine taste system. Therefore, the inventors embarked on a multi-faceted approach to develop knowledge of the porcine peripheral taste system in support of the project system development and the overall project goals of discovering taste modifiers.

[0120] In support of this work, microscopic assays were performed to ascertain the anatomy and physiology of the porcine taste bud system. Thes...

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Abstract

The present invention provides a method for determining cellular response to stimuli. The cells to be tested, for example, may be contained in a section of taste-bud containing lingual epithelium.

Description

RELATED APPLICATION [0001] This patent document is a continuation of U.S. application Ser. No. 11 / 313,418, filed on Dec. 21, 2005, which claims the benefit of priority of U.S. application Ser. No. 60 / 639,152, filed Dec. 22, 2004, and PCT application number PCT / US2005 / 41946 filed on Nov. 17, 2005, which applications are herein incorporated by reference.BACKGROUND OF THE INVENTION [0002] Taste transduction is one of the most sophisticated forms of chemotransduction in animals. Gustatory signaling is found throughout the animal kingdom, from simple metazoans to the most complex vertebrates. Its main purpose is to provide a reliable signaling response to non-volatile ligands. Humans typically distinguish several perceptual taste qualities or modalities: sweet, sour, salty, bitter and umami. Each of these modalities is thought to be mediated by distinct signaling pathways mediated by receptors or channels, leading to receptor cell depolarization, generation of a receptor or action potent...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/53
CPCG01N33/5008G01N33/502G01N33/5058G01N33/5088Y10T436/25375G01N33/6872G01N2500/00Y10T436/13G01N33/68
Inventor PAUL KIM, CHIN HONGMCDONALD, JOHNSMITH, SEAN S.ANDERSON, BRIAN B.MUROSKI, ALLEN R.GUTHRIE, BRIANST. JEOR, VAR LENTEETER, JOHN H.RAWSON, NANCY E.
Owner PAUL KIM CHIN HONG
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