Three-dimensional nanostructured hybrid scaffold and manufacture thereof
a nanostructured, hybrid technology, applied in the direction of artificial cell construction, prosthesis, coating, etc., can solve the problems of reducing the mechanical stability of the scaffold, catching air, and limited use of isolated biological materials
Inactive Publication Date: 2014-11-06
AARHUS UNIV +1
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Benefits of technology
The solution provides improved mechanical stability and cell mobility within the scaffold, facilitating effective nutrient and waste transport, thereby supporting cell growth and differentiation, and maintaining structural integrity during preparation, insertion, and use.
Problems solved by technology
An important issue in tissue regeneration and repair is the fabrication of three-dimensional scaffolds in such a manner that they mimic the extracellular matrix and thereby encourage the cells to grow functional tissues and allow the diffusion of nutrients, metabolites and soluble factors.
However, the usage of such isolated biological materials is limited because of insufficient mechanical properties upon implantation and during perfusion cell seeding.
However, an increase in the porosity of the resulting foam decreases the mechanical stability of the scaffold.
WO2006093778 states that the problem with retaining the foam within the structure is that it traps air and may be restrictive to cell migration and travel.
Therefore, the foam cannot be of the polymer type disclosed in the paper by Heijkants et al.
The hollow structure of the braided tube makes it especially vulnerable towards compressive forces under preparation, insertion and use.
Method used
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VI. Definitions
[0045]Prior to discussing the present invention in further details, the following terms and conventions will first be defined:
[0046]Solid freeform fabrication (SFF) is a collection of techniques for manufacturing solid objects by the sequential delivery of energy and / or material to specified points in space to produce a solid. SFF is sometimes referred to as rapid prototyping, rapid manufacturing, layered manufacturing and additive fabrication.
[0047]Solvent Casting—Particulate Leaching (SCPL) allows the preparation of porous structures with regular porosity, but with a limited scaffold size. First the polymer is dissolved into a suitable organic solvent (e.g. polylactic acid could be dissolved into dichloromethane), and then the solution is cast into a mould filled with porogen particles. Such porogen can be an inorganic salt like sodium chloride, crystals of saccharose, gelatin spheres or paraffin spheres. The size of the porogen particles will affect the size of the...
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A method of making a three-dimensional biocompatible scaffold capable of supporting cell activities such as growth and differentiation, the method includes providing a supporting grid that forms an open network and provides mechanical support of a second biocompatible material. The second biocompatible material has interconnected cavities that allow nutrients, metabolites and soluble factors to diffuse throughout the scaffold. The scaffold design can be understood as a hierarchically organised structure. At the micron to submicron length scale a top / down manufacturing approach is used to make a structure that will constitute the frame into which a bottom / up processing approach is applied to form an open porous scaffold with specific nano sized features. The advantage of this hierarcially organised design is that benefits can be drawn independently from both the micron and the nano sized structures, promoting specific cell activities and providing sufficient mechanical compliance.
Description
CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 13 / 377,430 filed on Jan. 31, 2012, which is a U.S. National Phase Application of PCT International Application Number PCT / DK2010 / 050167, filed on Jun. 25, 2010, designating the United States of America and published in the English language, which is an International Application of and claims the benefit of priority to European Patent Application No. 09163896.5, filed on Jun. 26, 2009. The disclosures of the above-referenced applications are hereby expressly incorporated by reference in their entireties.I. TECHNICAL FIELD OF THE INVENTION[0002]The present invention relates to three-dimensional hybrid scaffolds for tissue engineering and methods of their manufacture and use.II. BACKGROUND OF THE INVENTION[0003]Tissue engineering is a strategy for repairing or regenerating tissue. Cell culture in the context of tissue engineering further requires a three-dimensional scaffold for...
Claims
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IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2533/20C12N2533/50A61L27/34A61L27/56
InventorNYGAARD, JENS VINGEBJERRE, LEABUNGER, CODY ERICBESENBACHER, FLEMMING
OwnerAARHUS UNIV