Liposomes for inhibiting biofilm formation
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Inhibition of P. aeruginosa Multi-Drug Resistant Strain 6077 Biofilm
[0150]A preferred inventive mixture of empty liposomes proved to decrease biofilm formation by Pseudomonas aeruginosa strain 6077, as revealed by the Crystal violet method. Said preferred inventive mixture of empty liposomes consists of a 1:1 (weight per weight—w / w) mixture of said first empty liposomes and said second liposomes, wherein said first empty liposome is composed of a 1:1 weight ratio (1:1 w / w; 35:65 molar ratio) of sphingomyelin (SM) and cholesterol (CHOL), and said second empty liposomes is composed exclusively of SM.
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[0151]Briefly, Pseudomonas aeruginosa strain 6077 was grown in Trypticase Soy Broth (TSB) and diluted in LB broth containing 2% glucose to a concentration of 1×107 or of 1×106 colony forming units (CFU) per mL. One hundred microliters (100 μl) of the diluted inoculum was added to the wells of a 96-well flat-bottomed microtiter plate. Increasing concentrations of preferred inventive m...
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