Application of spiraea alpine extract in antiviral drug preparation

A technology of Alpine meadowsweet and antiviral drug, applied in the field of biomedicine, can solve the problems such as no reports on research on antibacterial and antiviral effects of Alpine meadowsweet, and achieve the effects of preventing virus invasion and protecting cells

CN103610705AInactive Publication Date: 2014-03-05黄瑞雪
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2014-03-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a novel application of spiraea alpine extract, and the novel application is the application of spiraea alpine extract in the antiviral drug preparation. The spiraea alpine extract is prepared by the following steps: taking spiraea alpine as a raw material, extracting volatile oil from the spiraea alpine, then extracting the volatile oil with ethanol with a concentration of 30% to 75%, and finally purifying the extract with a macroporous absorption resin and neutral alumina so as to obtain the finished product. The spiraea alpine extract provided by the invention has a very important meaning for antivirus drug preparation.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and relates to the application of an extract of traditional Chinese medicinal materials in the preparation of antiviral drugs, in particular to the application of the extract of Spiraea alpine in the preparation of antiviral drugs. Background technique

[0002] The increasing bacterial resistance caused by the extensive use of antibiotics has become one of the problems of common concern all over the world. Especially the drug resistance rate of Staphylococcus aureus has reached more than 90%. Herpes simplex virus type II mainly causes skin and mucous membrane infection in the genital area. The virus enters the body through the respiratory tract, oral cavity, genital mucosa and damaged skin, and resides in the normal mucosa, blood, saliva and sensory ganglion cells of the human body. When the body's resistance declines, such as fever, gastrointestinal disorders, menstruation, pregnancy, focal infectio...

Examples

Embodiment 1

[0035] Embodiment 1: the preparation of alpine Spiraea extract

[0036] (1) Spiraea alpine 18kg, extracted with 95% ethanol, filtered to obtain extract A and dregs A, extract A was concentrated to an alcohol-free paste, added distilled water, steam distilled, and collected the distillate , adding 4% of distillate weight β-cyclodextrin clathrate, cold storage, filtration, low-temperature drying, to obtain the clathrate of the volatile oil of Spiraea alpine;

[0037](2) Use the dregs A obtained in step (1) with 60% ethanol solution as a solvent, the extraction temperature is 80°C, the number of extractions is 2 times, the extraction time is 2 hours each time, and the amount of solvent used each time is Meadowsweet alpine 12 times the weight; filter to obtain extract B and drug residue B; extract B, recover ethanol, concentrate to relative density d=1.13, filter to obtain drug C;

[0038] (3) Pass the drug solution C obtained in step (2) through ADS-3 macroporous adsorption resi...

Embodiment 2

[0041] Embodiment 2: the preparation of alpine Spiraea extract

[0042] (1) 15kg of Spiraea alpine, extract with 95% ethanol, filter to obtain extract A and dregs A, extract A is concentrated to an alcohol-free paste, add distilled water, carry out steam distillation, and collect the distillate , adding 4% of distillate weight β-cyclodextrin clathrate, cold storage, filtration, low-temperature drying, to obtain the clathrate of the volatile oil of Spiraea alpine;

[0043] (2) Use the dregs A obtained in step (1) with 30% ethanol solution as a solvent, the extraction temperature is 95°C, the number of extractions is 1 time, and the extraction time is 4 hours each time, and the amount of solvent used each time is Spiraea alpine 15 times the weight; filter to obtain extract B and drug residue B; extract B, recover ethanol, concentrate to relative density d=1.18, filter to obtain drug C;

[0044] (3) Pass the medicinal liquid C obtained in step (2) through HP20 macroporous adsorp...

Embodiment 3

[0047] Example 3: Preparation of Spiraea Alpine Extract and Monomer Compounds

[0048] (1) 20kg of Spiraea alpine, extract with 95% ethanol, filter to obtain extract A and dregs A, concentrate extract A to a clear paste without alcohol, add distilled water, carry out steam distillation, and collect the distillate , adding 4% of distillate weight β-cyclodextrin clathrate, cold storage, filtration, low-temperature drying, to obtain the clathrate of the volatile oil of Spiraea alpine;

[0049] (2) Use the dregs A obtained in step (1) with 75% ethanol solution as a solvent, the extraction temperature is 50°C, the number of extractions is 3 times, and the extraction time is 1 hour each time, and the amount of solvent used each time is Meadowsweet alpine 6 times the weight; filter to obtain extract B and medicinal residue B; extract B, recover ethanol, concentrate to relative density d=1.10, filter to obtain medicinal liquid C;

[0050] (3) Pass the drug solution C obtained in step...