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The application discloses a method for separating lithium from high magnesium-lithium ratio salt lake brine, comprising the following steps: S1, mixing gas and liquid in a nanobubble generator to obtain nanobubble liquid by the nanobubble generator; S2, separating lithium and magnesium; pumping the nanobubble liquid obtained in the last step into a nanobubble separator, and making the nanobubbles rise with the liquid in a cylinder; due to the quantum characteristics of the nanobubbles, chemical reactions and ion exchanges occur in the contact process, the heavier magnesium rises with the nanobubbles, and the lighter lithium remains in the liquid phase, and then the lithium ions are collected by a lithium ion collecting unit. The process flow of the application is as follows: first, nanobubble liquid is prepared by the nanobubble generator, the nanobubbles rise with the liquid in the cylinder, the heavier magnesium rises with the nanobubbles, and the lighter lithium remains in the liquid phase, and then the lithium ions are collected by the lithium ion collecting unit, so that the separation of lithium and magnesium in the salt lake brine is realized.