Process for producing pyruvic acid utilizing dermoid hyphomycete yeast fermentation

A technology of Trichosporium dermatosa and pyruvate, which is applied in the biological field and can solve the problems of high production costs

Inactive Publication Date: 2007-08-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the method for above-mentioned production pyruvic acid all needs to add multivitamin and amino acid in culture medium, and production cost is higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Trichosporium dermatitis 9070 (CGMCC 0754) was inoculated in 30ml fermentation medium 1 contained in a 250ml shake flask, and the medium components were glucose 20%, peptone 5%, KH 2 PO 4 0.5%, MgSO 4 0.1%, CaCO 3 5%. After fermenting at 30° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 40.1 g / L of pyruvate.

Embodiment 2

[0009] Trichosporium dermatitis 9070 (CGMCC 0754) was inoculated in 25ml fermentation medium 2 contained in a 250ml shake flask, and the medium components were glucose 5%, beef extract 5%, KH 2 PO 4 0.1%, MgSO 4 0.02%, CaCO 3 1%. After fermenting at 24°C for 1 day, take 10ml of fermentation broth and inoculate 75ml of fermentation medium 3 in a 250ml shaker flask. 2 PO 4 0.15%, MgSO 4 0.07%, CaCO 3 5%. After fermenting at 28° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 42.3 g / L of pyruvate.

Embodiment 3

[0011] Trichosporium dermidis 9070 (CGMCC 0754) was inoculated in 50ml fermentation medium 4 contained in a 250ml shake flask, and the medium components were molasses 8%, corn steep liquor 2%, KH 2 PO 4 0.05%, MgSO 4 0.01%, CaCO 3 1%. After fermenting at 32° C. for 1 day, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 32.8 g / L of pyruvate.

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Abstract

Pyruvic acid is the important intermediary metabolite in biologic sugar metabolism. It has sanitarian effect and is the material of synthesizing products such as L- tryptophan, L-tyrosine and L- DOPA. This invention is a kind of method of producing pyruvic acid by dermoid hyphomycosis yeast to ferment glucose. It can get more than 40g per litre pyruvic acid from simple culture medium without adding vitamin and amino acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and is a new process for producing organic acids by yeast fermentation. Specifically, the present invention relates to a method for fermentatively producing pyruvate using Trichosporium dermatosa yeast. Background technique [0002] Pyruvic acid is an important intermediate metabolite of sugar metabolism in organisms, and has health-care and physiological functions (Pyruvic acid's health function. Soft Drinks International, 1998 (5): 24.), and is the synthesis of L-tryptophan, L-tyrosine , L-dopa and other products of raw materials. Pyruvate can be obtained by microbial fermentation of glucose (Qiang Hua, Chen Yang, kazuyuki Shimizu: J. Biosci. Bioeng.. 1999, 87 (2): 206-213; A. Yokota, H. Shimizu, Y. Terasawa et al: Appl .Microbiol.Biotech..1994, 41:638-643; Mitsuaki Moriguchi: Agric.Biol.Chem..1982, 46(4):955-961; M.Moriguchi, K.Shuto, T.Hashimoto: J.Ferment .Technol..1984, 62(3):243-248; S.Taka...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/40C12R1/85
Inventor 江宁王钦宏沈安贺鹏卢大军
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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