Molecular marking closely linked with wheat Brock powdery mildew resistant gene

Abstract

This is the molecular mark that closely linked with resistance genes to powdery mildew in wheat Brock. It involves the AFLP molecular mark and used primer that linked with resistance genes to powdery mildew. To find the molecular mark that linked more closely with unknown resistance gene of powdery mildew, we use Brock and Jing411 as well as near isogenic lines combined Brock and Jing411 as material to filter the mark by 225 AFLP primer combination. Among them, the primer combination P7 / M14 generates 227bp specific fragment, which has strong linkage with resistance gene, and named as P7 / M14227. The genetic distance between this mark and resistance gene is 1.9cM. Then cloning and sequencing P7 / M14227 to get the real length is 227bp. It can be used as new molecular mark of resistance genes to powdery mildew in Brock. This mark is more likely to be used in breeding.

Description

technical field [0001] The invention belongs to agricultural biotechnology engineering, in particular to an AFLP molecular marker linked with a powdery mildew resistance gene and a primer used therefor. technical background [0002] Wheat powdery mildew caused by the obligate parasitic fungus Erysiphe graminis f.sp.tritici is one of the main diseases that endanger wheat production. In recent years, with the promotion of dwarf and semi-dwarf wheat varieties, the increase of field planting density and the improvement of water and fertilizer conditions, the disease has become more and more serious in the northern and southern wheat regions of my country, and has become one of the main obstacles to high and stable wheat yields. one. In 1990, wheat powdery mildew was prevalent in 39% of the country's wheat growing areas, resulting in an output loss of 400 million kilograms. At present, wheat powdery mildew has become one of the common diseases that threaten wheat production in m...

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Problems solved by technology

It has been reported that Brock contains Pm2 (Liu Z Y, Sun Q X, Ni Z F, et al. 1999. Development of SCAR markers linked to the Pm21 gene conferring resistance topowdery mildew in common wheat. Plant Breeding, 118: 215-219), but Most of the wheat varieties containing pm2 in production have lost resistance to powdery mildew (Li Longye, Huang Yuanjiang. The effect and evaluation of known resistance genes to wheat powdery mildew. Chinese Agricultural Sciences, 1990, 23(3): 20-26)

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