Molecular marker linked to bolting resistance gene of head cabbage and its obtaining method

Abstract

The invention discloses a molecular marker linked with a bolting resistance gene of head cabbage and an obtaining method thereof. In this method, the F1 population was obtained by crossing the bolting-easy material and the bolting-resistant material first, and then the F2 population was constructed by self-crossing; the F2 population of heading cabbage was screened for bolting-resistant molecular markers by using the SSR molecular marker technology and the improved BSA method, and the heading cabbage was obtained. Molecular marker BolSSR00207 closely linked to bolting resistance gene. The genetic distance between the SSR molecular marker and the gene controlling bolting resistance is 10.69cM, and it is a (CT)n dinucleotide repeat type, which is (CT)8 in bolting-prone plants and (CT)10 in bolting-resistant plants . 34 randomly selected head cabbage varieties with different bolting properties were verified, and the coincidence rate reached 82.35%. The research results provide a molecular marker-assisted selection method for bolting-resistant cabbage, and lay a research foundation for the isolation of new bolting-resistant genes in cabbage. Theoretical research on bolting resistance is of great significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker BolSSR00207 linked with a bolting resistance gene of head cabbage (Brassica oleracea L. var. capitata L.) and an obtaining method thereof. Background technique [0002] Cabbage is the basic species of Brassica "U triangle" crops with CC diploid genome, including 8 cultivated cabbage, cauliflower, broccoli, Brussels sprouts, kale, kohlrabi, savoy cabbage and kale. variant. There is a high rate of natural hybridization among varieties. Brassica vegetables are currently widely cultivated all over the world. Heading cabbage has the largest cultivation area. Heading cabbage, cauliflower, broccoli and kohlrabi are commonly cultivated in my country, and Chinese kale is mainly cultivated in South China and other places. According to reports, in 2013, the annual planting area of ​​head cabbage in my country was about 900,000 hm 2 ; The planting area of ​​broc...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned major problems affecting the sustainable development of cruciferous crops in my country, and to discover new bolting-resistant germplasm resources, in the past, breeders mostly used morphological markers and biochemical markers to assist breeding, and some excellent bolting-resistant germplasm resources have been obtained after screening. Inbred lines, but in the process of bolting-resistant transformation, the traditional method of screening bolting-resistant plants has disadvantages such as long selection time and cumbersome operating procedures; on the other hand, the performance of bolting resistance is easily affected by environmental conditions. Difficulty adapting to the needs of breeding practice

Images