Flax powdery mildew disease-resistant gene molecule marker

Abstract

The invention belongs to a molecule mark of a flax powdery mildew disease resistance gene, belonging to the agricultural biotechnology engineering, in particular the molecule mark which is interlocked with flax powdery mildew disease resistance gene and a method for obtaining the same. The invention solves the problems of high workload, easy effect of environment and long breeding period and so on of the normal mark method. The method comprises the following steps of: undergoing resistance screening to a flax powdery mildew disease resistance material (9801-1) and an infected material by adopting a RAPD mark technology; researching genetic regularity of the flax powdery mildew disease resistance to screen flax powdery mildew disease resistance material and the infected material to obtain the molecule mark which is interlocked with the flax powdery mildew disease resistance gene, namely 0PP0 (792). The mark is used for selecting breeding under the aid of powdery mildew disease resistance flax, laying a foundation of cloning new powdery mildew disease resistance gene at the same time, and laying a good foundation of cloning flax powdery mildew disease resistance gene, analyzing the gene sequence and researching the transferred powdery mildew disease resistance gene flax.

Description

technical field [0001] The invention belongs to the fields of flax disease-resistant breeding and molecular biology, and particularly relates to a molecular marker linked with a flax powdery mildew resistance gene and an obtaining method, which can be applied to molecular marker assisted breeding. Background technique [0002] At present, powdery mildew is a common disease in flax producing areas in my country and one of the main diseases that endanger flax production. It occurs in the field for a short period and spreads quickly. In recent years, according to surveys in Heilongjiang, Yunnan, and Xinjiang, flax has occurred throughout the field in the late growth period, and there are few disease-resistant varieties, especially foreign varieties. The causative agent of flax powdery mildew is Oidium Lini Skoric (flax powdery mildew), which belongs to the fungus of the subphylum Deuteromycotina; the behavioral state is Erysiphe cichoracearum DC. The powdery mildew pathogen in...

AI Technical Summary

Problems solved by technology

[0004] At present, the molecular genetic map of flax is not complete, and only linkage groups containing 13 RFLP and 80 RAPD markers have been established [8]

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