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Molecular Marker Linked to Rice Drought Tolerance Gene qlri9 and Its Application

A rice and drought tolerance technology, which is applied in the field of agricultural biotechnology engineering, can solve the problem that it is difficult to greatly improve the drought tolerance level of rice, and achieve the effect of overcoming a long time period and strong reliability.

Inactive Publication Date: 2020-05-08
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Genetic studies have shown that rice drought tolerance is a complex quantitative trait. The drought tolerance response involves multiple genes, and there is an obvious interaction effect between genes and the environment. It is difficult to improve the drought tolerance level of rice by relying on traditional breeding methods. greatly improved
With the development of molecular biology, it has become possible to use molecular assisted breeding for agronomic traits that are difficult to identify and are easily affected by the environment. This technology can perform accurate and stable selection in the early generations, thereby accelerating the breeding process and improving breeding efficiency. The basis of this technology is to have an excellent target gene and its closely linked molecular markers

Method used

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  • Molecular Marker Linked to Rice Drought Tolerance Gene qlri9 and Its Application
  • Molecular Marker Linked to Rice Drought Tolerance Gene qlri9 and Its Application
  • Molecular Marker Linked to Rice Drought Tolerance Gene qlri9 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. Acquisition of Rice Drought Tolerance Gene qLRI9 and Its Closely Linked Molecular Markers

[0049] 1. Mapping of Drought Tolerance QTL and Acquisition of Rice Drought Tolerance Gene qLRI9

[0050] 1. Test materials

[0051]The F 10 The recombinant inbred line population (RIL) was used as the targeting population.

[0052] 2. Genotype identification

[0053] The test materials were planted in the Beijing Changping Experimental Base, and the young rice leaves were clipped, and the genome-wide DNA extraction was carried out according to Doyle and Dickson (1987) with a slightly improved method of cetyltriethylammonium bromide (CTAB). 178 pairs of SSR primers with good amplification effect, polymorphism between parents and uniform distribution in 12 linkage groups were screened out for genotype identification of the mapping population.

[0054] The PCR reaction system is as follows: 10×PCR buffer (containing Mg 2+ ) 1.0μl, 10mM dNTP Mixture 0.25μl, 10pM / μl pr...

Embodiment 2

[0085] Embodiment 2, rice drought tolerance identification method

[0086] The method for identifying the drought tolerance of rice to be tested provided by the invention is as follows:

[0087] 1. Extract the genomic DNA of the rice to be tested, use the genomic DNA as a template, and perform PCR amplification with the RM24598 molecular marker to obtain a PCR product;

[0088] The PCR reaction system is as follows: 10×PCR buffer (containing Mg 2+ ) 1.0μl, 10mM dNTP Mixture 0.25μl, 10pM / μl primer 0.25μl, 0.5U / μl Taq polymerase 0.25μl, 40ng / μl genomic DNA 1.0μl, ddH 2 O to make up to 10 μl.

[0089] The PCR reaction conditions were as follows: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 40 s, 36 cycles; extension at 72°C for 10 min.

[0090] 2. Identify the drought tolerance of the rice to be tested according to the size of the PCR product:

[0091] If the size of the PCR product is 110bp, the rice to ...

Embodiment 3

[0096] Example 3, Application of RM24598 Molecular Marker in the Identification of Rice Variety Drought Tolerance

[0097] 1. Test materials

[0098] The tested materials were Huakewagu, Dalisha, Yunchuanbai, Xiaomizao, Taipei 8, Huangbansuo, Heidagu, Jinxing 1, Guichao 2 and Manpixianghong glutinous rice.

[0099] 2. Identification of Drought Tolerance

[0100] According to the method in Step 3 of Example 1, the drought tolerance of the test materials in Step 1 was identified.

[0101]The results are shown in Table 4. It can be seen from the table that among the 10 rice varieties, Taipei No. 8 and Huangbansuo have a leaf curl degree of 7, both of which are rice varieties with weak drought tolerance, and Jinxing No. 1 has a leaf curl degree of 5, which indicates drought tolerance. Among the rice varieties, the remaining 7 rice varieties Huakewagu, Dalisha, Yunchuanbai, Xiaomizao, Heidagu, Guichao No. 2 and Manpixianghong glutinous rice are all drought-tolerant. Strong rice...

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Abstract

The invention discloses a molecular marker linked with a drought-resistant rice gene qLRI9, and application thereof in rice drought resistance identification. Two sets of rice-dry rice recombinant inbred lines (RIL) with different genetic backgrounds are utilized for successfully positioning a genetic locus qLRI9 relevant to rice drought resistance onto a rice No.9 chromosome, drought-resistant beneficial alleles come from a drought-resistant parent IAPAR-9 and are linked with the molecular marker RM24598, and the method for identifying or assistant identifying rice drought resistance is provided based on the molecular marker RM24598 and can be used for identifying a rice drought resistance variety and molecular breeding.

Description

technical field [0001] The invention belongs to agricultural biotechnology engineering, and specifically relates to a molecular marker linked with rice drought tolerance gene qLRI9 and its application. Background technique [0002] The problem of land drought is widespread in the world, and the arable land area in arid and semi-arid regions has accounted for 42.9% of the global arable land area. According to statistics, more than 60 million hectares of rice planting areas in the world are under drought stress every year, and the resulting yield loss is staggering. In recent years, droughts that have spread widely and lasted for a long time often occur in my country, and the number of years affected by drought is also increasing year by year. Drought has gradually become a prominent problem affecting agricultural production, and it has a tendency to worsen. The resulting reduction in production is far greater than the sum of the reductions caused by other environmental factor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 崔迪韩龙植马小定王娇李亚非韩冰孙建昌
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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