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Method for cultivating pest-resistant wheat and its special-purpose gene

A wheat and gene technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as laborious and time-consuming

Inactive Publication Date: 2009-01-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Using a series of traditional genetic methods such as interspecific hybridization, backcrossing, and selective breeding to breed new varieties with insect-resistant traits is time-consuming and laborious, and can only introduce alleles of the same or related species into the species to be improved. Among the crops

Method used

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  • Method for cultivating pest-resistant wheat and its special-purpose gene
  • Method for cultivating pest-resistant wheat and its special-purpose gene
  • Method for cultivating pest-resistant wheat and its special-purpose gene

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, cultivating aphid-resistant wheat

[0033] 1. Construction of plant expression vector pBAC202

[0034]Codon optimization was carried out on the GNA gene, and sGNA was artificially synthesized (in order to distinguish it from the GNA gene, the codon-optimized synthesis genes were respectively named sGNA). sGNA has the nucleotide sequence of sequence 1 in the sequence listing and encodes the amino acid of sequence 2 in the sequence listing. The amino acid sequence is identical to the sequence of GNA encoded by the GNA gene.

[0035] For the construction method of the plasmid and the subsequent screening and identification process, refer to the literature (Sambrook, J, Fritsch, E.F, Maniatis, T, Molecular Cloning-A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.). The specific method is as follows:

[0036] 1) Use SacI and EcoRI to double-digest plasmid pSP72 (purchased from Promega), and insert about 260 bp of Agrobacteriu...

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Abstract

The invention discloses a method for cultivating pest-resistant wheat and dedicated gene, which has one of the following nucleic acid sequences, (1)nucleic acid sequence of SEQ ID No:1 in the sequence table, (2) nucleic acid sequence capable of hybridizing with the nucleic acid sequence of SEQ No.1 in the sequence table under highly stringent conditions.

Description

technical field [0001] The invention relates to a method for cultivating insect-resistant wheat and its special gene. Background technique [0002] As an important cereal crop, wheat is the main source of food for the world's population. In my country, wheat is the second largest food crop, and aphids are one of the important pests in wheat production. In recent years, with the improvement of water and fertilizer conditions in wheat fields and the change of agricultural ecological environment, wheat aphids have become more and more serious, and have become an important obstacle that seriously limits the high, stable and high-quality wheat yield. [0003] Using a series of traditional genetic methods such as interspecific hybridization, backcrossing, and selective breeding to breed new varieties with insect-resistant traits is time-consuming and laborious, and can only introduce alleles of the same or related species into the species to be improved. among the crops. There ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82
Inventor 张晓东陈绪清梁荣奇杨凤萍张立全高泽发
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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