Method and technique of the separated tissue culture of Kava

A technology of tissue culture and kava, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of no regenerated kava plants, achieve large commercial application prospects, and overcome technical bottlenecks Effect

Inactive Publication Date: 2007-09-26
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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Problems solved by technology

Taylor and Taufa tried it in 1998, but it didn't work
In 2001, Briskin et al. used various methods such as surface disinfection and adding antibiotics to the culture medium, but only obtained calli from leaves and stems of kava pepper. So far, no complete regeneration of kava has been obtained through in vitro tissue culture technology. Pepper plant reports

Method used

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  • Method and technique of the separated tissue culture of Kava

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Embodiment Construction

[0021] The present invention is described in detail below in conjunction with embodiment:

[0022] 1. Source of material: The annual stems of Kava pepper (Piper Methysticum Forst.f) introduced from Cook and other South Pacific island countries and planted in the greenhouse of the State Key Laboratory of Tropical Crops Biotechnology, Chinese Academy of Tropical Agricultural Sciences were used as the initial explants.

[0023] 2. Method:

[0024] (1) Material pretreatment and explant disinfection ① Pretreatment: take healthy annual stems for pretreatment, rinse with streamlined tap water overnight or not less than 12 hours; ② Explant disinfection: cut the initial explant 1 cm long stem explants, each explant (stem) with 1 dormant bud, treated with 70% alcohol for 30-60 seconds, sterilized with 0.1% mercury liter for 10-15 minutes, rinsed with sterile water for 3- 4 times, 5 minutes each time.

[0025] (2) Induce dormant buds to sprout

[0026] Inoculate the sterilized explant...

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Abstract

The invention relates to a slip pepper isolated organism cultivating technique and method, which uses two kinds of organism cultivate techniques to realize the slip pepper regeneration. The invention is characterized in that it overcomes the technique bottleneck of slip pepper isolated organism cultivation as endotrophic mycorrhiza pollution, with which the slip pepper can be cultivated via various methods, to realize the regeneration and quick cultivation of slip pepper and harmless seed via germ cultivation and callus induction. The slip pepper seed cultivated by the invention is germ free and harmless while th cultivate index in each month can reach 7-8, the root rate can reach 100%, and the transplant survival rate can reach 87.2%. The invention has significant social and economic benefits.

Description

[technical field] [0001] The invention relates to a technique and a method for in vitro tissue culture of kava, including two ways of bud propagation and callus induction to successfully realize the regeneration and rapid propagation of sterile and non-toxic kava seedlings. [Background technique] [0002] Kava pepper (Piper Methysticum Forst.f), commonly known as Kava, is a perennial shrub of the family Piperaceae (Piperaceae), a well-known medicinal plant, mainly distributed in Vanuatu, Fiji, Tonga, Papua— South Pacific island countries such as New Guinea and the Solomon Islands have been introduced to my country for trial planting in recent years. [0003] Kava pepper has been used medicinally and cultivated in the South Pacific island countries for more than 3,000 years. Beverages prepared from kava roots or rhizomes have the effect of relaxing muscles and emotions, restoring energy, promoting sleep, and alleviating pain. The analysis of pharmacological components shows ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G1/00A01G7/00
Inventor 张治礼陈雄庭郑学勤
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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