Platelets from stem cells

A technology for platelets and human embryonic stem cells, applied in the field of platelets derived from stem cells, can solve problems such as insufficient proof

Inactive Publication Date: 2007-10-10
WISCONSIN ALUMNI RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this idea is not well documented, and platelet formation and isolation from megakaryocytes are still areas of urgent research

Method used

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Embodiment

[0027] hematopoietic precursor cells of embryoid bodies

[0028] Embryoid body (EB) formation is a method used to study the hematopoietic differentiation of mouse and human ES cells. However, unlike mouse ES cells, single cell suspensions of human ES cells do not form embryoid bodies efficiently. However, for embryoid body formation from human ES cells, intact colonies of human ES cells cultured on mouse embryonic fibroblasts (MEFs) were digested with 0.5 mg / ml dispase for 5 minutes to form small cell colonies. These cell colonies were then further agglutinated in serum-containing stem cell medium (20% FCS). Embryoid bodies began to form after 6 days of incubation of readily distinguishable cell clumps with single cells that did not participate in the formation of cell clumps but apoptotic. After 12 days of culture, the embryoid bodies assembled into early yolk sac embryo structures. Sections of the embryoid bodies were made and subsequently immunolabeled with CD34 antibody...

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Abstract

Human embryonic stem cells are induced to differentiate first into the hematopoietic lineage and then into megakaryocytes, the cells which generate platelets. The proper in vitroculture of megakaryocytes results in the production and shed of platelets. This makes possible, for the first time, the in vitro production of a human blood factor needed by many patients.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Applications Serial No. 60 / 623,922, filed November 1, 2004 and Serial No. 60 / 714,578, filed September 7, 2005. [0003] Statement Regarding Federally Sponsored Research or Development [0004] To be determined. Background of the invention [0005] Stem cells are defined as cells capable of differentiating into many other differentiated cell types. Embryonic stem cells are stem cells derived from embryos that are capable of differentiating into most, if not all, of the differentiated cell types of the mature organism. Stem cells are called pluripotent, which describes the ability of these cells to differentiate into many cell types. One class of pluripotent stem cells of great interest to the research community are human embryonic stem cells, sometimes written hES or human ES cells, ie embryonic stem cells deri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/078
Inventor J・A・托马森D・陈
Owner WISCONSIN ALUMNI RES FOUND
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