Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Platelets from stem cells

A technology for platelets and human embryonic stem cells, applied in the field of platelets derived from stem cells, can solve problems such as insufficient proof

Inactive Publication Date: 2007-10-10
WISCONSIN ALUMNI RES FOUND
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this idea is not well documented, and platelet formation and isolation from megakaryocytes are still areas of urgent research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Platelets from stem cells
  • Platelets from stem cells
  • Platelets from stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] hematopoietic precursor cells of embryoid bodies

[0028] Embryoid body (EB) formation is a method used to study the hematopoietic differentiation of mouse and human ES cells. However, unlike mouse ES cells, single cell suspensions of human ES cells do not form embryoid bodies efficiently. However, for embryoid body formation from human ES cells, intact colonies of human ES cells cultured on mouse embryonic fibroblasts (MEFs) were digested with 0.5 mg / ml dispase for 5 minutes to form small cell colonies. These cell colonies were then further agglutinated in serum-containing stem cell medium (20% FCS). Embryoid bodies began to form after 6 days of incubation of readily distinguishable cell clumps with single cells that did not participate in the formation of cell clumps but apoptotic. After 12 days of culture, the embryoid bodies assembled into early yolk sac embryo structures. Sections of the embryoid bodies were made and subsequently immunolabeled with CD34 antibody...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Human embryonic stem cells are induced to differentiate first into the hematopoietic lineage and then into megakaryocytes, the cells which generate platelets. The proper in vitroculture of megakaryocytes results in the production and shed of platelets. This makes possible, for the first time, the in vitro production of a human blood factor needed by many patients.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Applications Serial No. 60 / 623,922, filed November 1, 2004 and Serial No. 60 / 714,578, filed September 7, 2005. [0003] Statement Regarding Federally Sponsored Research or Development [0004] To be determined. Background of the invention [0005] Stem cells are defined as cells capable of differentiating into many other differentiated cell types. Embryonic stem cells are stem cells derived from embryos that are capable of differentiating into most, if not all, of the differentiated cell types of the mature organism. Stem cells are called pluripotent, which describes the ability of these cells to differentiate into many cell types. One class of pluripotent stem cells of great interest to the research community are human embryonic stem cells, sometimes written hES or human ES cells, ie embryonic stem cells deri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/078
Inventor J・A・托马森D・陈
Owner WISCONSIN ALUMNI RES FOUND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com