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Sulfate reduction bacterium with polyacrylamide degradation function and application thereof

A polyacrylamide, sulfate technology, applied in applications, bacteria, biochemical equipment and methods, etc., can solve problems such as few microbial reports

Active Publication Date: 2008-01-16
江苏哈宜环保研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finding microorganisms that can produce reducing substances, and promoting the chain oxidation degradation reaction of polyacrylamide, so as to complete the degradation of polyacrylamide, is an important way to solve the discharge of polyacrylamide-containing sewage. At present, there are few microbial reports on the degradation of polyacrylamide.

Method used

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  • Sulfate reduction bacterium with polyacrylamide degradation function and application thereof
  • Sulfate reduction bacterium with polyacrylamide degradation function and application thereof
  • Sulfate reduction bacterium with polyacrylamide degradation function and application thereof

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specific Embodiment approach 1

[0015] Specific embodiment 1: In this embodiment, the sulfate-reducing bacteria with polyacrylamide degradation function is named as Salmonella Daqing MF, which is Salmonella Daqing MF, and its taxonomic genus is Salmonella, and its taxonomic species is Salmonella Daqing. It was named as Salmonella Daqing, and it was preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.1910 and the preservation date being January 10, 2007.

specific Embodiment approach 2

[0016] Specific embodiment two: the separation of the Salmonella Daqing MF strain in this embodiment is realized by the following method: take 5-10 mL of the polymer aqueous solution in the aging tank of the polymer injection station, and put it into a chamber containing nitrogen gas with a purity of 99.99%. Add 3 to 5 glass beads to an anaerobic bottle of oxygen sterile water, shake on a shaker for 1.5 hours, break up the bacterial micelles in the polymer solution, and dilute the bacteria with anaerobic sterile water. Concentration diluted to 10 6 , then inoculated in solid medium, and then "rolled tube" cultured for 7-10 days; picked 1-3 rings of single colonies and transferred them into liquid medium, and then separated and purified 3-6 times, confirmed with electron microscope and Isolate the pure strains, expand the pure strains and configure them into a bacterial solution, in which the bacterial concentration is configured as 10 6 ; configure different concentrations of...

specific Embodiment approach 3

[0018] Specific embodiment three: in the present embodiment, the identification of Salmonella Daqing MF bacterial classification is realized by the following methods: 1, the Salmonella Daqing MF bacterium among the present invention is Gram-negative short bacillus, and the colony form of Salmonella Daqing MF is as shown in Figure 1, When growing on a solid medium, it is a black round colony with a smooth surface and irregular edges; 2. Figure 2 is a morphological diagram of Salmonella Daqing MF observed with an atomic force microscope. The colony has a capsule, and the length of the bacteria is variable. In the range of 0.35 μm to 0.7 μm in width × 0.7 μm to 1.6 μm in length, the bacteria have no flagella; 3. Classification and identification of Salmonella Daqing MF strains: DNA of the strain is extracted with a kit (produced by Treasure Biotechnology Co., Ltd.), and universal primers are used For PCR amplification, primer Eubac27F for 16S rDNA sequence: 5'-AGAGTTTGATCCTGGCTCAG...

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Abstract

Disclosed are a sulfate reducing bacterium with a polyacylamide degradation function and applications of the bacterium. The invention relates to a microorganism and applications of the microorganism. The invention firstly put forward the sulfate reducing bacterium with the polyacylamide degradation function, the bacterium is named as Salmonella Daqing MF, namely, Salmonella Daqing MF (preservation code: CGMCC No.1910), preserved in the China General Microbiological Culture Collection Center, and belonging to the Salmonella Genera and the Salmonella Daqing Species in taxonomy. The bacterium belonging to the Gram negative short-bacilli is covered by a capsule and is obligate anaerobic, variable in size bound in 0.35Mu-0.7Mum width multiplied by 0.7Mum-1.6Mum length, without flagella on the bacterium body; when growing on a solid medium, the bacterium develops into a black round colony, smooth in surface and irregular in edge.

Description

technical field [0001] The present invention relates to a kind of microorganism and its application. Background technique [0002] In oilfield tertiary oil recovery, water-soluble polymer (HPAM) has been widely used in all aspects of oilfield industry. Daqing Oilfield is now widely used as "three-element compound flooding" oil recovery technology, "three-element" mainly includes ultra-high molecular weight polyacrylamide (HPAM), alkali and surfactant. Ultra-high molecular weight polyacrylamide (HPAM) aqueous solution is used as an oil displacement agent to inject into the oil layer, so that the mobility ratio between crude oil with high viscosity (mainly saturated hydrocarbons, aromatics, colloids and asphaltenes) and water decreases. , Weaken the viscous fingering of water, thereby enhancing oil recovery. The produced fluid of polymer flooding contains not only crude oil but also polyacrylamide. It causes a large increase in the sewage containing polyacrylamide, and the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31C08L33/26C08J11/00
Inventor 马放魏利李维国
Owner 江苏哈宜环保研究院有限公司
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