Seedling budding method for rubber tree minitype bud sticks
A technology for budding of seedlings and rubber trees, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve problems such as inconvenience in picking and pruning buds, shorten the cultivation period, increase the output of dry glue, and cut early. The effect of production
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[0012] The following examples illustrate the present invention in further detail:
[0013] During the cultivation of rootstock: in the rubber seed maturation season, collect fresh seeds and germinate them on the sand bed for 18-22 days, select seedlings with a height of about 20-30 cm and in the stage of elongation to leaf expansion, and remove the seedlings from the sand bed Pull up, rinse with water to remove the sand at the base of the seedling stems and roots, and soak the roots of the seedlings in a container filled with shallow water for budding.
[0014] During the cultivation of micro-bud strips: first use rubber anthers as explants, add 2.4-D0.5-1mg / l to MS basic medium, kinetin 0.5-1mg / l, naphthaleneacetic acid 1-2mg / l, coconut water 5%, callus induced on medium with sucrose concentration of 70-100g / l; add kinetin 0.2-1mg / l, naphthaleneacetic acid 0.1-0.2mg / l, coconut water 5%, activated carbon 1g in MS basic medium / l, the sucrose concentration is 70g / l culture med...
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