High anticoagulating active antihuman tissue factor monoclone antibody, preparation method and application thereof

A monoclonal antibody, tissue factor technology, applied in biochemical equipment and methods, antibodies, tissue culture, etc., can solve the problem that no anti-TF monoclonal antibody has been reported, and achieve the effect of high-efficiency anticoagulant activity

Inactive Publication Date: 2008-06-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there have been no reports of anti-TF monoclonal antibodies with high anticoagulant activity

Method used

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  • High anticoagulating active antihuman tissue factor monoclone antibody, preparation method and application thereof
  • High anticoagulating active antihuman tissue factor monoclone antibody, preparation method and application thereof
  • High anticoagulating active antihuman tissue factor monoclone antibody, preparation method and application thereof

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Experimental program
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Embodiment Construction

[0017] The monoclonal antibody of the present invention recognizes human TF antigen.

[0018] The monoclonal antibody of the present invention can be produced by the hybridoma of the present invention, and thus can be obtained from a culture medium in which the hybridoma of the present invention is cultured or from ascites fluid of a mouse immunized with the hybridoma of the present invention. However, the method of producing the monoclonal antibody of the present invention is not particularly limited, and it is also within the scope of the present invention if the genetically engineered antibody can specifically bind to human TF.

[0019] The monoclonal antibody that recognizes the specific site of human TF produced by the hybridoma of the present invention can be obtained by fusing spleen cells or lymph node cells of animals immunized with TF-MAP with myeloma cells.

[0020] The hybridomas of the present invention can be produced using cell fusion techniques known in the art...

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PUM

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Abstract

The invention belongs to the biological technical field, in particular to a high anticoagulation activity human tissue factor monoclonal antibody, a relative preparation method and application thereof. The invention process bioinformatics and antigen immunogennicity analysis at the conjugate point of tissue factor and X factor, to search amino acid sequence with better immunogenicity, which contains key amino acid sequence with conjugated tissue factor and X factor, to prepare tissue factor multiple antigenic peptide, and uses the polypeptide immunized mice to prepare hybridoma cell to obtain high anticoagulation activity mice anti-human tissue factor monoclonal antibody which can be used in thrombus treatment and relative TF disease diagnosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an anti-human tissue factor monoclonal antibody with high anticoagulation activity and a preparation method and application thereof. Background technique [0002] In normal humans, the intravascular procoagulant and anticoagulant activities maintain an appropriate balance. Under the influence of atherosclerosis, hypertension, hyperlipidemia, diabetes and other pathological factors, the balance may be inclined to the direction of procoagulation, which will eventually lead to thrombosis, causing life-threatening acute myocardial infarction, acute cerebral infarction and acute pulmonary embolism. disease. Therefore, there is a need for effective and safe anticoagulants to treat specific thrombotic diseases. [0003] Tissue factor (TF) is a transmembrane glycoprotein, which is divided into three parts: extracellular domain, transmembrane domain and intracellular domain. Under normal phy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/18A61K39/395A61P7/02G01N33/53
Inventor 马端蔡旭刘静彭卓醇孔德升李笑天
Owner FUDAN UNIV
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